PHPT1 Protein (AA 1-125) (His tag)
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- 抗原 See all PHPT1 蛋白
- PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-125
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PHPT1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MAVADLALIP DVDIDSDGVF KYVLIRVHSA PRSGAPAAES KEIVRGYKWA EYHADIYDKV SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYSMAYGPA QHAISTEKIK AKYPDYEVTW ANDGY
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH7.5 at 37C.
- Top Product
- Discover our top product PHPT1 蛋白
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phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125) protein (GST tag)
PHPT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA, PAA
phosphohistidine Phosphatase 1 (PHPT1) (AA 2-125) protein (His tag)Crystallography grade PHPT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
phosphohistidine Phosphatase 1 (PHPT1) proteinPHPT1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125), (full length) protein (His tag)PHPT1 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
phosphohistidine Phosphatase 1 (PHPT1) (AA 1-125), (full length) protein (GST tag)PHPT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
phosphohistidine Phosphatase 1 (PHPT1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PHPT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.2M NaCl, 2 mM DTT, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PHPT1 (phosphohistidine Phosphatase 1 (PHPT1))
- 别名
- PHPT1 (PHPT1 产品)
- 别名
- wu:fb50e08 Protein, zgc:101086 Protein, MGC131298 Protein, PHP14 Protein, CGI-202 Protein, HSPC141 Protein, RP11-216L13.10 Protein, bA216L13.10 Protein, 1700008C22Rik Protein, Php14 Protein, phosphohistidine phosphatase 1 Protein, phosphohistidine phosphatase 1 L homeolog Protein, 14 kDa phosphohistidine phosphatase-like Protein, LOC424401 Protein, phpt1 Protein, PHPT1 Protein, phpt1.L Protein, LOC100511240 Protein, Phpt1 Protein
- 背景
- PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 15.9 kDa (145aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_054891
- UniProt
- Q9NRX4
- 途径
- Positive Regulation of Peptide Hormone Secretion
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