Lactate Dehydrogenase A Protein (LDHA) (AA 1-332) (His tag)
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- 抗原 See all Lactate Dehydrogenase A (LDHA) 蛋白
- Lactate Dehydrogenase A (LDHA)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-332
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宿主
- 大鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This Lactate Dehydrogenase A protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 200 units/mg, in which one unit will convert 1.0 umole of pyruvate to L-lactate and beta-NAD per minute at pH 7.5 at 37C.
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- Discover our top product LDHA 蛋白
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Lactate Dehydrogenase A (LDHA) (AA 283-332) protein (His-IF2DI Tag)
LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Lactate Dehydrogenase A (LDHA) (AA 5-323) protein (His tag)LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lactate Dehydrogenase A (LDHA) (AA 1-332) protein (GST tag)LDHA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lactate Dehydrogenase A (LDHA) (AA 1-332) (Active) protein (His tag)LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Lactate Dehydrogenase A (LDHA) protein (His tag)LDHA 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Lactate Dehydrogenase A (LDHA) (AA 5-323), (partial) protein (His tag)LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
Lactate Dehydrogenase A (LDHA) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)LDHA 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, STD, PI Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 1 mM DTT, 20 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- Lactate Dehydrogenase A (LDHA)
- 别名
- Ldha (LDHA 产品)
- 别名
- GSD11 Protein, LDH1 Protein, LDHM Protein, LDH-M Protein, ldh-h Protein, ldha Protein, ldha-B Protein, ldhbb Protein, trg-5 Protein, Ldh1 Protein, Ldhm Protein, l7R2 Protein, gsd11 Protein, ldh-m Protein, ldh1 Protein, ldhm Protein, pig19 Protein, ldha1 Protein, ldhab Protein, LDH-A Protein, LDHC Protein, lactate dehydrogenase A Protein, lactate dehydrogenase B L homeolog Protein, lactate dehydrogenase A4 Protein, lactate dehydrogenase A L homeolog Protein, L-lactate dehydrogenase A chain Protein, LDHA Protein, ldhb.L Protein, Ldha Protein, ldha Protein, ldha.L Protein, LOC100713875 Protein
- 背景
- Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Recombinant rat Ldha, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 37.5kDa (340aa) 28-40KDa (SDS-PAGE under reducing conditions)
- NCBI登录号
- NP_058721
- UniProt
- P04642
- 途径
- Warburg Effect
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