Lactate Dehydrogenase A Protein (LDHA) (Transcript Variant 1) (Myc-DYKDDDDK Tag)
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- 抗原 See all Lactate Dehydrogenase A (LDHA) 蛋白
- Lactate Dehydrogenase A (LDHA)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 1
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Lactate Dehydrogenase A protein is labelled with Myc-DYKDDDDK Tag.
- 应用范围
- Antibody Production (AbP), Functional Studies (Func), Standard (STD), Protein Interaction (PI)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human LDHA (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 80 % as determined by SDS-PAGE and Coomassie blue staining
- Biological Activity Comment
- Higher specific activity than endogenous human LDHA: OriGene human recombinant LDHA was compared side-by-side with purified human liver LDH5 in a spectrophotometric pyruvate to lactate conversion assay. Activity is shown as a decrease in absorbance at 340nm over time. The activity of recombinant human LDHA is comparable to that of endogenously expressed human LDH5.
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- Discover our top product LDHA 蛋白
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Lactate Dehydrogenase A (LDHA) (AA 283-332) protein (His-IF2DI Tag)
LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Lactate Dehydrogenase A (LDHA) (AA 5-323) protein (His tag)LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Lactate Dehydrogenase A (LDHA) (AA 1-332) protein (GST tag)LDHA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lactate Dehydrogenase A (LDHA) (AA 1-332) (Active) protein (His tag)LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Lactate Dehydrogenase A (LDHA) protein (His tag)LDHA 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Lactate Dehydrogenase A (LDHA) (AA 2-332) protein (His tag)Crystallography grade LDHA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase A (LDHA) (AA 2-332) protein (His tag)Crystallography grade LDHA 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lactate Dehydrogenase A (LDHA) (AA 5-323), (partial) protein (His tag)LDHA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 说明
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The tag is located at the C-terminal.
- 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- Lactate Dehydrogenase A (LDHA)
- 别名
- Ldha (LDHA 产品)
- 别名
- GSD11 Protein, LDH1 Protein, LDHM Protein, LDH-M Protein, ldh-h Protein, ldha Protein, ldha-B Protein, ldhbb Protein, trg-5 Protein, Ldh1 Protein, Ldhm Protein, l7R2 Protein, gsd11 Protein, ldh-m Protein, ldh1 Protein, ldhm Protein, pig19 Protein, ldha1 Protein, ldhab Protein, LDH-A Protein, LDHC Protein, lactate dehydrogenase A Protein, lactate dehydrogenase B L homeolog Protein, lactate dehydrogenase A4 Protein, lactate dehydrogenase A L homeolog Protein, L-lactate dehydrogenase A chain Protein, LDHA Protein, ldhb.L Protein, Ldha Protein, ldha Protein, ldha.L Protein, LOC100713875 Protein
- 背景
- The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.
- 分子量
- 36.5 kDa
- NCBI登录号
- NP_005557
- 途径
- Warburg Effect
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