MAP4K3 Protein (AA 1-321) (His tag)
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- 抗原 See all MAP4K3 蛋白
- MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-321
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宿主
- E. coli
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资源
- 大肠杆菌(E. Coli)
- 标记
- This MAP4K3 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL
- 纯度
- > 95 % by SDS - PAGE
- Biological Activity Comment
- Specific activity is > 70 units/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
- Top Product
- Discover our top product MAP4K3 蛋白
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer ( pH 8.0) containing 0.15M NaCl, 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- MAP4K3 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 3 (MAP4K3))
- 别名
- glk (MAP4K3 产品)
- 别名
- GLK Protein, MAPKKKK3 Protein, MEKKK 3 Protein, MEKKK3 Protein, RAB8IPL1 Protein, 4833416M01Rik Protein, 4833416M07Rik Protein, 9530052P13Rik Protein, Glk Protein, mitogen-activated protein kinase kinase kinase kinase 3 Protein, MAP4K3 Protein, Map4k3 Protein
- 背景
- Glk belongs to the bacterial glucokinase family. This protein is not highly important in E.coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E.coli glk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- 分子量
- 37.1kDa (344aa), confirmed by MALDI-TOF
- NCBI登录号
- NP_416889
- UniProt
- P0A6V8
- 途径
- MAPK Pathway
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