IMPAD1 Protein (AA 34-359) (His tag)
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- 抗原 See all IMPAD1 蛋白
- IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 34-359
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IMPAD1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK
- 纯度
- > 90 % by SDS - PAGE
- Biological Activity Comment
- Specific acitivty > 3300 pmole/min/ug, its ability to dephosphorylate adenosine 3'5'-diphosphate sodium slat at pH 7.5, 25C.
- Top Product
- Discover our top product IMPAD1 蛋白
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Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-359) protein (His tag)
IMPAD1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Inositol Monophosphatase Domain Containing 1 (IMPAD1) protein (His tag)IMPAD1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 1-359) protein (GST tag)IMPAD1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-356) protein (His tag)IMPAD1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-356) (Active) protein (His tag)IMPAD1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.25 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4)
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))
- 别名
- IMPAD1 (IMPAD1 产品)
- 别名
- IMP 3 Protein, RGD1306455 Protein, gPAPP Protein, impa3 Protein, 1110001C20Rik Protein, AA408880 Protein, AI451589 Protein, AL022796 Protein, B230207P20 Protein, Jaws Protein, GPAPP Protein, IMP-3 Protein, IMPA3 Protein, IMPase 3 Protein, zgc:123256 Protein, inositol monophosphatase domain containing 1 Protein, inositol monophosphatase domain containing 1 S homeolog Protein, Impad1 Protein, impad1.S Protein, IMPAD1 Protein, impad1 Protein
- 背景
- IMPAD1, also known as Inositol monophosphatase 3, is a member of the inositol monophosphatase family. IMPAD1 is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
- 分子量
- 37.6kDa (349aa) confirmed by MALDI-TOF
- NCBI登录号
- NP_060283
- UniProt
- Q9NX62
- 途径
- Glycosaminoglycan Metabolic Process
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