IMPAD1 Protein (AA 34-356) (His tag)
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- 抗原 See all IMPAD1 蛋白
- IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 34-356
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This IMPAD1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- ADPGRFSLFG LGSEPAAGEA EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP VLGVIHKPFS EYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 5,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze Adenosine 3, 5-diphosphate per minute at pH 7.5 at 25C.
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Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-359) protein (His tag)
IMPAD1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Inositol Monophosphatase Domain Containing 1 (IMPAD1) protein (His tag)IMPAD1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 1-359) protein (GST tag)IMPAD1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-356) protein (His tag)Crystallography grade IMPAD1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 1-359) protein (rho-1D4 tag)Crystallography grade IMPAD1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 1-356) protein (rho-1D4 tag)Crystallography grade IMPAD1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Inositol Monophosphatase Domain Containing 1 (IMPAD1) (AA 34-356) protein (His tag)IMPAD1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- IMPAD1 (Inositol Monophosphatase Domain Containing 1 (IMPAD1))
- 别名
- Impad1 (IMPAD1 产品)
- 别名
- IMP 3 Protein, RGD1306455 Protein, gPAPP Protein, impa3 Protein, 1110001C20Rik Protein, AA408880 Protein, AI451589 Protein, AL022796 Protein, B230207P20 Protein, Jaws Protein, GPAPP Protein, IMP-3 Protein, IMPA3 Protein, IMPase 3 Protein, zgc:123256 Protein, inositol monophosphatase domain containing 1 Protein, inositol monophosphatase domain containing 1 S homeolog Protein, Impad1 Protein, impad1.S Protein, IMPAD1 Protein, impad1 Protein
- 背景
- IMPAD1, also known as inositol monophosphatase 3, may play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3, 5-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Recombinant mouse IMPAD1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 36.2kDa (332aa) 28-40KDa (SDS-PAGE under non-reducing conditions.)
- NCBI登录号
- NP_808398
- UniProt
- Q80V26
- 途径
- Glycosaminoglycan Metabolic Process
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