ESM1 Protein (AA 20-184) (His tag)
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- 抗原 See all ESM1 蛋白
- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
- 生物活性
- Active
- 产品特性
- AA 20-184
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This ESM1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- Endothelial cell-specific molecule 1 (ESM1) is a proteoglycan secreted by endothelial cells (primarily in the human lung and kidney tissues) and its mRNA expression is regulated by inflammatory cytokines. Endocan expression, which detected in various epithelia and adipocytes has been shown to be upregulated by vascular endothelial growth factor (VEGF), fibroblast growth factor 2 (FGF-2), TNF alpha, IL1 beta, or lipopolysaccharide and downregulated by IFN gamma. Genetically engineered cells overexpressing ESM1 induce tumor formation, implying that ESM1 might be involved in the pathophysiology of tumor growth in vivo. Besides, Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) has been identified as an interactor of ESM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ESM1 and recombinant human LFA1a. Briefly, ESM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LFA1a-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti- ESM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of ESM1 and LFA1a was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of ESM1 with LFA1a.
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- Discover our top product ESM1 蛋白
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Endothelial Cell-Specific Molecule 1 (ESM1) protein (His tag)
ESM1 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Endothelial Cell-Specific Molecule 1 (ESM1) (AA 1-184) protein (GST tag)ESM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endothelial Cell-Specific Molecule 1 (ESM1) (AA 85-184) protein (GST tag)ESM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endothelial Cell-Specific Molecule 1 (ESM1) protein (His tag)ESM1 宿主: 人 宿主: 人细胞 Recombinant >90 % as determined by reducing SDS-PAGE.
Endothelial Cell-Specific Molecule 1 (ESM1) protein (His tag)ESM1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE
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- 应用备注
- Isoelectric Point: 6.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))
- 别名
- Endothelial Cell Specific Molecule 1 (ESM1) (ESM1 产品)
- 别名
- ESM1 Protein, zgc:153027 Protein, 0610042H23Rik Protein, AV004503 Protein, ESM-1 Protein, Pg25 Protein, endocan Protein, endothelial cell specific molecule 1 Protein, endothelial cell-specific molecule 1 Protein, ESM1 Protein, esm1 Protein, Esm1 Protein
- 背景
- Alternative Names: Endocan
- 分子量
- 19kDa
- UniProt
- Q9NQ30
- 途径
- Growth Factor Binding
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