PLG Protein (AA 191-433) (His tag)
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- 抗原 See all PLG 蛋白
- PLG (Plasminogen (PLG))
- 生物活性
- Active
- 产品特性
- AA 191-433
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PLG protein is labelled with His tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of Plg with ACTb.
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Plasminogen (PLG) (AA 98-356) protein (His tag)
PLG 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Plasminogen (PLG) (AA 21-120) protein (GST tag)PLG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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Plasminogen (PLG) (AA 100-352) protein (His tag)PLG 宿主: 人 宿主: Yeast (Pichia pastoris) Recombinant > 95 % WB, SDS, ELISA, IP
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- 应用备注
- Isoelectric Point: 7.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- PLG (Plasminogen (PLG))
- Abstract
- PLG 产品
- 别名
- wu:fb70e09 Protein, PLG Protein, LPA Protein, plg Protein, Ab1-346 Protein, AI649309 Protein, Pg Protein, plasminogen Protein, plg Protein, PLG Protein, Plg Protein
- 背景
- Alternative Names: PL, Plasmin, Activation peptide, Angiostatin
- 分子量
- 38kDa
- UniProt
- Q01177
- 途径
- Complement System, Lipid Metabolism
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