GSTM1 Protein (AA 1-218) (His tag)
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- 抗原 See all GSTM1 蛋白
- GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))
- 生物活性
- Active
- 产品特性
- AA 1-218
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This GSTM1 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- Glutathione S-transferase Mu 1 (GSTM1) belongs to the mu class. GSTM1 can detoxifying carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Besides, uncoupling Protein 1 (UCP1) has been identified as an interactor of GSTM1, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat GSTM1 and recombinant rat UCP1. Briefly, GSTM1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCP1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-GSTM1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C . Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of GSTM1 and UCP1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of GSTM1 with UCP1.
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Glutathione S-Transferase mu 1 (GSTM1) (AA 1-218) protein
GSTM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Glutathione S-Transferase mu 1 (GSTM1) protein (His tag)GSTM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by reducing SDS-PAGE.
Glutathione S-Transferase mu 1 (GSTM1) (AA 1-100) protein (GST tag)GSTM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Glutathione S-Transferase mu 1 (GSTM1) (AA 1-218) (Active) protein (His tag)GSTM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Glutathione S-Transferase mu 1 (GSTM1) (AA 1-181) protein (GST tag)GSTM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Glutathione S-Transferase mu 1 (GSTM1) (AA 2-218) protein (His tag)Crystallography grade GSTM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 应用备注
- Isoelectric Point: 8.3
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- GSTM1 (Glutathione S-Transferase mu 1 (GSTM1))
- 别名
- Glutathione S Transferase Mu 1 (GSTm1) (GSTM1 产品)
- 别名
- GST1 Protein, GSTM1-1 Protein, GSTM1a-1a Protein, GSTM1b-1b Protein, GTH4 Protein, GTM1 Protein, H-B Protein, MU Protein, MU-1 Protein, GSTA3 Protein, Gstb-1 Protein, Gstb1 Protein, gst-mu Protein, gst1 Protein, gstm1 Protein, gstm2 Protein, gth4 Protein, gtm1 Protein, glutathione S-transferase mu 1 Protein, glutathione S-transferase M1 Protein, glutathione S-transferase, mu 1 Protein, glutathione S-transferase Mu 1 Protein, glutathione S-transferase mu 2 (muscle) Protein, glutathione S-transferase mu 1 L homeolog Protein, GSTM1 Protein, Gstm1 Protein, LOC100356307 Protein, GSTM2 Protein, LOC100731426 Protein, gstm1.L Protein
- 背景
- Alternative Names: GST-M1, GST1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, GTH4, GTM1, H-B, MU1, GST HB subunit 4
- 分子量
- 27kDa
- UniProt
- P04905
- 途径
- Negative Regulation of Transporter Activity
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