EIF2AK2 Protein (AA 224-502) (His tag)
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- 抗原 See all EIF2AK2 蛋白
- EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
- 生物活性
- Active
- 产品特性
- AA 224-502
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This EIF2AK2 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 80 %
- Biological Activity Comment
- Protein Kinase R (PKR) is activated by double-stranded RNA (dsRNA), the synthesis of which is caused virally. PKR can also be activated by the protein PACT or by heparin. It plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Besides, Cyclin Dependent Kinase 1 (CDK1) has been identified as an interactor of PKR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PKR and recombinant human CDK1. Briefly, PKR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CDK1-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PKR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of PKR and CDK1 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of PKR with CDK1.
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- Discover our top product EIF2AK2 蛋白
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Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 252-551) (Active) protein (GST tag)
EIF2AK2 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) protein (GST tag)EIF2AK2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 2-551) protein (His tag)Crystallography grade EIF2AK2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 2-515) protein (His tag)Crystallography grade EIF2AK2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) proteinEIF2AK2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)EIF2AK2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 8.9
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
- 别名
- Protein Kinase R (PKR) (EIF2AK2 产品)
- 别名
- PKR Protein, pkr Protein, EIF2AK2 Protein, DKFZp469K1934 Protein, EIF2AK1 Protein, PRKR Protein, 2310047A08Rik Protein, 4732414G15Rik Protein, AI467567 Protein, AI747578 Protein, Pkr Protein, Prkr Protein, Tik Protein, eukaryotic translation initiation factor 2 alpha kinase 2 Protein, eukaryotic translation initiation factor 2-alpha kinase 2 Protein, EIF2AK2 Protein, eif2ak2 Protein, Eif2ak2 Protein
- 背景
- Alternative Names: PRKR, PRK-R, EIF2AK2, EIF2AK1, Eukaryotic Translation Initiation Factor 2 Alpha Kinase 2, Interferon-induced, double-stranded RNA-activated protein kinase, P1/eIF-2A kinase
- 分子量
- 32kDa
- UniProt
- P19525
- 途径
- DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C
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