FGF22 Protein (AA 26-162) (His tag)
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- 抗原 See all FGF22 蛋白
- FGF22 (Fibroblast Growth Factor 22 (FGF22))
- 生物活性
- Active
- 产品特性
- AA 26-162
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This FGF22 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- FGF22 (Fibroblast growth factor 22) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF22 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF22. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF22 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF22 for 48h. The result was shown in Figure 2. It was obvious that FGF22 significantly increased cell viability of 3T3 cells.
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Fibroblast Growth Factor 22 (FGF22) (AA 23-170) protein (His tag)
FGF22 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 22 (FGF22) (AA 23-170) protein (His-IF2DI Tag)FGF22 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Fibroblast Growth Factor 22 (FGF22) (Active) proteinFGF22 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Fibroblast Growth Factor 22 (FGF22) (AA 23-162) protein (His tag)Crystallography grade FGF22 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 11.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- FGF22 (Fibroblast Growth Factor 22 (FGF22))
- Abstract
- FGF22 产品
- 别名
- si:dkey-21e2.2 Protein, si:dkeyp-74c5.3 Protein, 2210414E06Rik Protein, FGF-22 Protein, fibroblast growth factor 22 Protein, FGF22 Protein, fgf22 Protein, Fgf22 Protein
- 分子量
- 20kDa
- UniProt
- Q9ESS2
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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