FGF23 Protein (AA 25-251) (His tag)
-
- 抗原 See all FGF23 蛋白
- FGF23 (Fibroblast Growth Factor 23 (FGF23))
- 生物活性
- Active
- 产品特性
- AA 25-251
-
宿主
- 小鼠
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This FGF23 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- FGF23 (Fibroblast growth factor 23) is a member of the fibroblast growth factor family, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. A proliferation assay was conducted to detect the bioactivity of recombinant mouse FGF23 using 3T3 cells. Briefly, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of FGF23. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of 3T3 cells after incubation with FGF23 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant FGF23 for 48h. The result was shown in Figure 2. It was obvious that FGF23 significantly increased cell viability of 3T3 cells.
-
Fibroblast Growth Factor 23 (FGF23) (AA 25-251) protein (His tag)
FGF23 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Fibroblast Growth Factor 23 (FGF23) (AA 1-251) protein (GST tag)FGF23 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Fibroblast Growth Factor 23 (FGF23) (AA 25-179) protein (His tag)Crystallography grade FGF23 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fibroblast Growth Factor 23 (FGF23) (AA 180-251) protein (His tag)Crystallography grade FGF23 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fibroblast Growth Factor 23 (FGF23) (AA 25-251) protein (His tag)Crystallography grade FGF23 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fibroblast Growth Factor 23 (FGF23) (AA 25-251) protein (His tag)Crystallography grade FGF23 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Fibroblast Growth Factor 23 (FGF23) (AA 25-251), (full length) protein (His tag)FGF23 宿主: 大鼠 宿主: 酵母菌 Recombinant > 90 % ELISA
-
- 应用备注
- Isoelectric Point: 9.8
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
-
- 抗原
- FGF23 (Fibroblast Growth Factor 23 (FGF23))
- Abstract
- FGF23 产品
- 别名
- FGF23 Protein, ADHR Protein, FGFN Protein, HPDR2 Protein, HYPF Protein, PHPTC Protein, fibroblast growth factor 23 Protein, fgf23 Protein, FGF23 Protein, Fgf23 Protein
- 背景
- Alternative Names: ADHR, HYPF, HPDR2, PHPTC, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor
- 分子量
- 29kDa
- UniProt
- Q9EPC2
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion
-
