Tissue factor Protein (AA 30-252) (His tag)
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- 抗原 See all Tissue factor (F3) 蛋白
- Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
- 生物活性
- Active
- 产品特性
- AA 30-252
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Tissue factor protein is labelled with His tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 95 %
- Biological Activity Comment
- TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of TF with EGF.
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Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 33-251) protein (His tag)
F3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 35-248) protein (His tag)F3 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 33-251) proteinF3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant WB, SDS, ELISA, IHC, DB
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 31-251) protein (His tag)F3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 45-154) protein (GST tag)F3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) protein (His tag)F3 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) protein (His tag)F3 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (Active) protein (His tag)F3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 33-251) protein (His tag)Crystallography grade F3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Coagulation Factor III (thromboplastin, Tissue Factor) (F3) (AA 29-251) protein (His tag)Crystallography grade F3 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Isoelectric Point: 9.2
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
- 别名
- Tissue Factor (TF) (F3 产品)
- 别名
- CD142 Protein, TF Protein, TFA Protein, PRO1557 Protein, PRO2086 Protein, TFQTL1 Protein, f3 Protein, AA409063 Protein, Cf-3 Protein, Cf3 Protein, tf Protein, zgc:112151 Protein, coagulation factor III, tissue factor Protein, transferrin Protein, coagulation factor IIIa Protein, coagulation factor III Protein, tissue factor Protein, coagulation factor IIIb Protein, coagulation factor III (thromboplastin, tissue factor) S homeolog Protein, F3 Protein, TF Protein, f3a Protein, tf Protein, f3b Protein, f3.S Protein
- 背景
- Alternative Names: CD142, FIII, F3, TFA, Thromboplastin, Coagulation Factor III
- 分子量
- 29kDa
- UniProt
- P42533
- 途径
- Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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