Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
蛋白类型
Recombinant
产品特性
AA 33-251
宿主
人
资源
HEK-293 Cells
标记
This Tissue factor protein is labelled with His tag.
原理
Human Coagulation Factor III/Tissue Factor Protein
序列
Ser33-Glu251
产品特性
Recombinant Human Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Glu251.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human Coagulation Factor III, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.
Crystallography grade
F3
宿主: 人
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
WB, SDS, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
Tissue factor (F3)
(Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
分子量
25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.