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Tissue factor Protein (AA 33-251) (His tag)

F3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
产品编号 ABIN7275700
发货至: 中国
  • 抗原 See all Tissue factor (F3) 蛋白
    Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
    蛋白类型
    Recombinant
    产品特性
    AA 33-251
    宿主
    • 15
    • 7
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    资源
    • 13
    • 13
    • 3
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    • 1
    HEK-293 Cells
    标记
    This Tissue factor protein is labelled with His tag.
    原理
    Human Coagulation Factor III/Tissue Factor Protein
    序列
    Ser33-Glu251
    产品特性
    Recombinant Human Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Glu251.
    纯度
    > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
    过滤
    0.22 μm filtered
    内毒素水平
    Less than 1EU per μg by the LAL method.
    Biological Activity Comment
    Immobilized Human Coagulation Factor III, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA. See testing image for detail.
  • 限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
    缓冲液
    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
    储存条件
    -20 °C,-80 °C
    储存方法
    -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
    有效期
    12 months
  • 抗原
    Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))
    别名
    Coagulation Factor III (F3 产品)
    别名
    CD142 Protein, TF Protein, TFA Protein, PRO1557 Protein, PRO2086 Protein, TFQTL1 Protein, f3 Protein, AA409063 Protein, Cf-3 Protein, Cf3 Protein, tf Protein, zgc:112151 Protein, coagulation factor III, tissue factor Protein, transferrin Protein, coagulation factor IIIa Protein, coagulation factor III Protein, tissue factor Protein, coagulation factor IIIb Protein, coagulation factor III (thromboplastin, tissue factor) S homeolog Protein, F3 Protein, TF Protein, f3a Protein, tf Protein, f3b Protein, f3.S Protein
    背景
    Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
    分子量
    25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
    途径
    Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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