Vitamin D Receptor Protein (VDR) (AA 272-427) (His tag)
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- 抗原 See all Vitamin D Receptor (VDR) 蛋白
- Vitamin D Receptor (VDR)
- 生物活性
- Active
- 产品特性
- AA 272-427
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Vitamin D Receptor protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- Vitamin D Receptor (VDR) is the nuclear hormone receptor for vitamin D3. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. It mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Besides, E1A binding protein p300 (EP300) has been identified as an interactor of VDR, thus a binding ELISA assay was conducted to detect the interaction of recombinant human VDR and recombinant human EP300. Briefly, VDR were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-VDR pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C . Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of of VDR and EP300 was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of VDR with EP300.
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- Discover our top product VDR 蛋白
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Vitamin D Receptor (VDR) (AA 1-427) protein (GST tag)
VDR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vitamin D Receptor (VDR) (AA 318-427) protein (GST tag)VDR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vitamin D Receptor (VDR) protein (His tag)VDR 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 86 % as determined by SDS-PAGE
Vitamin D Receptor (VDR) (AA 1-427) protein (Strep Tag)Crystallography grade VDR 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Vitamin D Receptor (VDR) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)VDR 宿主: 化学剂 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Vitamin D Receptor (VDR) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)VDR 宿主: 化学剂 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Isoelectric Point: 6.5
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 100 mMNaHCO3, 500 mMNaCl, pH 8.3, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- Vitamin D Receptor (VDR)
- Abstract
- VDR 产品
- 别名
- vdrbeta Protein, vdr0 Protein, LOC100136219 Protein, NR1I1-B Protein, gb:dq017633 Protein, vdr-b Protein, Ci-VDR-b Protein, VDR Protein, LOC100221284 Protein, vdr-A Protein, xVDR Protein, Nr1i1 Protein, vdr Protein, NR1I1 Protein, PPP1R163 Protein, vitamin D (1,25- dihydroxyvitamin D3) receptor Protein, vitamin D receptor Protein, vitamin D3 receptor A Protein, vitamin D receptor b Protein, nuclear receptor VDR-b Protein, vitamin D (1,25- dihydroxyvitamin D3) receptor L homeolog Protein, vitamin D (1,25-dihydroxyvitamin D3) receptor Protein, vitamin D receptor a Protein, vdr Protein, vdr0 Protein, VDR Protein, LOC100136219 Protein, vdrb Protein, vdr-b Protein, vdr.L Protein, Vdr Protein, vdra Protein
- 物质类
- Chemical
- 背景
- Alternative Names: NR1I1, Nuclear Receptor Subfamily 1 Group I Member 1, Calcitriol Receptor, 1,25-dihydroxyvitamin D3 receptor
- 分子量
- 20kDa
- UniProt
- P11473
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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