JAK2 Protein (AA 508-800) (His tag)
JAK2
宿主: 人
宿主: 大肠杆菌（E. Coli）
> 90 %
AcA, CC
Active
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- 抗原 See all JAK2 蛋白
- JAK2 (Janus Kinase 2 (JAK2))
- 生物活性
- Active
- 产品特性
- AA 508-800
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This JAK2 protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 90 %
- Biological Activity Comment
- JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was shown in Figure 1, and this effect was in a dose dependent manner The binding activity of JAK2 with EPOR.
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- Discover our top product JAK2 蛋白
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Janus Kinase 2 (JAK2) (AA 241-351) protein (His-IF2DI Tag)
JAK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Janus Kinase 2 (JAK2) (AA 1014-1132) protein (His tag)JAK2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
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- 应用备注
- Isoelectric Point: 6.7
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- JAK2 (Janus Kinase 2 (JAK2))
- Abstract
- JAK2 产品
- 别名
- JAK2bCA Protein, jak2 Protein, JTK10 Protein, THCYT3 Protein, AI504024 Protein, C81284 Protein, Fd17 Protein, JAK-2 Protein, Janus kinase 2b Protein, Janus kinase 2 Protein, jak2b Protein, JAK2 Protein, Jak2 Protein
- 背景
- Alternative Names: JTK10, Tyrosine-protein kinase JAK2
- 分子量
- 35kDa
- UniProt
- O60674
- 途径
- JAK/STAT Signaling, RTK signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response
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