NOG Protein (AA 28-144) (MBP tag,His tag)
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- 抗原 See all NOG 蛋白
- NOG (Noggin (NOG))
- 生物活性
- Active
- 产品特性
- AA 28-144
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This NOG protein is labelled with MBP tag,His tag.
- 应用范围
- Cell Culture (CC), Activity Assay (AcA)
- 产品特性
- Tag location: Two N-terminal Tags, His-tag and MBP-tag
- 纯度
- > 97 %
- Biological Activity Comment
- NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner The binding activity of NOG with BMP4.
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- Discover our top product NOG 蛋白
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Noggin (NOG) (AA 20-232) (Active) protein
NOG 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95% as determined by Tris-Bis PAGE
Noggin (NOG) (AA 28-232) (Active) proteinNOG 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) protein (His tag)NOG 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) (AA 28-232) protein (His tag)NOG 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Noggin (NOG) (AA 28-232) protein (GST tag)NOG 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Noggin (NOG) (AA 28-232) protein (Fc Tag)NOG 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- 应用备注
- Isoelectric Point: 6.6
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- NOG (Noggin (NOG))
- Abstract
- NOG 产品
- 别名
- SYM1 Protein, SYNS1 Protein, nog-A Protein, nog1 Protein, noggin-1 Protein, noggin Protein, noggin Protein, noggin L homeolog Protein, noggin protein Protein, NOG Protein, Nog Protein, nog.L Protein, noggin Protein
- 背景
- Alternative Names: SYM1, SYNS1, Synostoses(Multiple)Syndrome 1, Symphalangism 1(Proximal)
- 分子量
- 64kDa
- UniProt
- Q62809
- 途径
- Stem Cell Maintenance, Tube Formation
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