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IL-2 Protein (AA 21-169) (His tag)

IL2 宿主: 小鼠 宿主: HEK-293 Cells > 97 % AcA, CC Active
产品编号 ABIN6239755
发货至: 中国
  • 抗原 See all IL-2 (IL2) 蛋白
    IL-2 (IL2) (Interleukin 2 (IL2))
    生物活性
    Active
    产品特性
    AA 21-169
    宿主
    • 29
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    小鼠
    资源
    • 41
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    HEK-293 Cells
    标记
    This IL-2 protein is labelled with His tag.
    应用范围
    Activity Assay (AcA), Cell Culture (CC)
    产品特性
    Tag location: N-terminal His Tag
    纯度
    > 97 %
    Biological Activity Comment
    Interleukin-2 (IL-2), the main lymphokine produced by activated CD4+ T-helper lymphocytes, is a potent T-cell growth regulator. In addition to this activity, IL-2 was found to decrease the proliferation of 3T3 fibroblasts in time- and dose-dependent manner. Thus 3T3 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of IL2 for 48h, then 3T3 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. The inhibitory effect of IL-2 on cell proliferation of 3T3 cells observed by inverted microscope was shown in Figure 1.
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    Discover our top product IL2 蛋白
  • 应用备注
    Isoelectric Point: 4.9
    限制
    仅限研究用
  • 状态
    Lyophilized
    缓冲液
    20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
    储存液
    Dithiothreitol (DTT), Other preservative, ProClin
    注意事项
    This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
  • 抗原
    IL-2 (IL2) (Interleukin 2 (IL2))
    Abstract
    IL2 产品
    别名
    IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein
    背景
    Alternative Names: TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
    分子量
    25kDa
    UniProt
    P04351
    途径
    JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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