SMAD3 Protein (AA 2-425) (DYKDDDDK Tag,His tag)
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- 抗原 See all SMAD3 蛋白
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-425
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SMAD3 protein is labelled with DYKDDDDK Tag,His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Mass Spectrometry (MS)
- 原理
- Recombinant Human SMAD3 Protein is produced by E.coli expression system and the target gene encoding Ser2-Ser425 is expressed with a 6His, Flag tag at the N-terminus.
- 序列
- MRGSHHHHHH GSDYKDDDDK SSILPFTPPI VKRLLGWKKG EQNGQEEKWC EKAVKSLVKK LKKTGQLDEL EKAITTQNVN TKCITIPRSL DGRLQVSHRK GLPHVIYCRL WRWPDLHSHH ELRAMELCEF AFNMKKDEVC VNPYHYQRVE TPVLPPVLVP RHTEIPAEFP PLDDYSHSIP ENTNFPAGIE PQSNIPETPP PGYLSEDGET SDHQMNHSMD AGSPNLSPNP MSPAHNNLDL QPVTYCEPAF WCSISYYELN QRVGETFHAS QPSMTVDGFT DPSNSERFCL GLLSNVNRNA AVELTRRHIG RGVRLYYIGG EVFAECLSDS AIFVQSPNCN QRYGWHPATV CKIPPGCNLK IFNNQEFAAL LAQSVNQGFE AVYQLTRMCT IRMSFVKGWG AEYRRQTVTS TPCWIELHLN GPLQWLDKVL TQMGSPSIRC SSVS
- 纯度
- Greater than 95 % as determined by reducing SDS-PAGE.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
- Top Product
- Discover our top product SMAD3 蛋白
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SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-425) protein (His tag)
SMAD3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 33-178) protein (His-IF2DI Tag)SMAD3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 1-110) protein (GST tag)SMAD3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 31-141) protein (GST tag)SMAD3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 120-221) protein (GST tag)SMAD3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (AA 147-270) protein (GST tag)SMAD3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
SMAD, Mothers Against DPP Homolog 3 (SMAD3) protein (GST tag,His tag)SMAD3 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE
SMAD, Mothers Against DPP Homolog 3 (SMAD3) (Transcript Variant 1) (Active) protein (Myc-DYKDDDDK Tag)SMAD3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, pH 7.5.
- 注意事项
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
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- 抗原
- SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))
- 别名
- SMAD3 (SMAD3 产品)
- 别名
- HSPC193 Protein, HsT17436 Protein, JV15-2 Protein, LDS1C Protein, LDS3 Protein, MADH3 Protein, madh3 Protein, madh3a Protein, smad3 Protein, wu:fa99e03 Protein, XSmad3 Protein, XenMLP Protein, Xmad3 Protein, madh3-A Protein, Madh3 Protein, AU022421 Protein, Smad 3 Protein, mad3 Protein, madh3b Protein, zgc:92234 Protein, SMAD family member 3 Protein, SMAD family member 3a Protein, SMAD family member 3 L homeolog Protein, SMAD family member 3b Protein, SMAD3 Protein, smad3a Protein, smad3.L Protein, Smad3 Protein, smad3b Protein
- 基因ID
- 4088
- UniProt
- P84022
- 途径
- Cell Division Cycle, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy
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