RNASE3 Protein (AA 28-160) (His tag)
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- 抗原 See all RNASE3 (ECP) 蛋白
- RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))
- 蛋白类型
- Recombinant
- 产品特性
- AA 28-160
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This RNASE3 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- ADPRPPQFTR AQWFAIQHIS LNPPRCTIAM RAINNYRWRC KNQNTFLRTT FANVVNVCGN QSIRCPHNRT LNNCHRSRFR VPLLHCDLIN PGAQNISNCR YADRPGRRFY VVACDNRDPR DSPRYPVVPV HLDTTIHHHH HH
- 纯度
- > 95 % by SDS-PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Top Product
- Discover our top product ECP 蛋白
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))
- 别名
- RNASE3 (ECP 产品)
- 别名
- ECP Protein, RNS3 Protein, ribonuclease A family member 3 Protein, RNASE3 Protein
- 背景
- RNASE3, also known as Ribonuclease RNase A family 3, located in the eosinophil primary matrix. It released during degranulation of eosinophils. This protein is related to inflammation and asthma because in these cases, there are increased levels of ECP in the body. It is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Recombinant human RNASE3 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 16.6kDa (142aa) 18-28KDa (SDS-PAGE under reducing conditions.)
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