VCAM1 Protein (AA 25-698) (His tag)
-
- 抗原 See all VCAM1 蛋白
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 25-698
-
宿主
- 人
-
资源
- Baculovirus infected Insect Cells
- 标记
- This VCAM1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- ADPEFFKIET TPESRYLAQI GDSVSLTCST TGCESPFFSW RTQIDSPLNG KVTNEGTTST LTMNPVSFGN EHSYLCTATC ESRKLEKGIQ VEIYSFPKDP EIHLSGPLEA GKPITVKCSV ADVYPFDRLE IDLLKGDHLM KSQEFLEDAD RKSLETKSLE VTFTPVIEDI GKVLVCRAKL HIDEMDSVPT VRQAVKELQV YISPKNTVIS VNPSTKLQEG GSVTMTCSSE GLPAPEIFWS KKLDNGNLQH LSGNATLTLI AMRMEDSGIY VCEGVNLIGK NRKEVELIVQ EKPFTVEISP GPRIAAQIGD SVMLTCSVMG CESPSFSWRT QIDSPLSGKV RSEGTNSTLT LSPVSFENEH SYLCTVTCGH KKLEKGIQVE LYSFPRDPEI EMSGGLVNGS SVTVSCKVPS VYPLDRLEIE LLKGETILEN IEFLEDTDMK SLENKSLEMT FIPTIEDTGK ALVCQAKLHI DDMEFEPKQR QSTQTLYVNV APRDTTVLVS PSSILEEGSS VNMTCLSQGF PAPKILWSRQ LPNGELQPLS ENATLTLIST KMEDSGVYLC EGINQAGRSR KEVELIIQVT PKDIKLTAFP SESVKEGDTV IISCTCGNVP ETWIILKKKA ETGDTVLKSI DGAYTIRKAQ LKDAGVYECE SKNKVGSQLR SLTLDVQGRE NNKDYFSPEH HHHHH
- 纯度
- > 90 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of U937 Human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates 10ug/ml. This effect is more to 70%.
- Top Product
- Discover our top product VCAM1 蛋白
-
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
-
Bioactivity Validated
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
-
- 抗原
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 别名
- VCAM1 (VCAM1 产品)
- 别名
- CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein
- 背景
- VCAM1, also known as vascular cell adhesion protein 1 isoform, a member of the immunoglobulin superfamily. It is a cell surface protein expressed by activated endothelial cells and certain leukocytes. VCAM1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection and asthmatic responses. During the inflammatory adhesion mechanism, activated integrins that rolling leukocytes and attach them firmly to the vascular endothelium. Recombinant human VCAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 75.6kDa (685aa) 70-100KDa (SDS-PAGE under reducing conditions.)
- NCBI登录号
- NP_001069
- UniProt
- P19320
- 途径
- Carbohydrate Homeostasis
-