VCAM1 Protein (His tag)
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- 抗原 See all VCAM1 蛋白
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This VCAM1 protein is labelled with His tag.
- 原理
- Recombinant Mouse VCAM1 Protein (His Tag)(Active)
- 序列
- Met 1-Glu 698
- 产品特性
- A DNA sequence encoding the extracellular domain (Met 1-Glu 698) of mouse VCAM1 (NP_035823.3) was fused with the a polyhistidine tag at the C-terminus.
- 纯度
- > 97 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour at RT.
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- Discover our top product VCAM1 蛋白
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Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-697) protein (His tag)
VCAM1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Vascular Cell Adhesion Molecule 1 (VCAM1) protein (Fc Tag)VCAM1 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by reducing SDS-PAGE.
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-309) protein (His tag)VCAM1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Cell Adhesion Molecule 1 (VCAM1) protein (His tag)VCAM1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % by SDS - PAGE SDS
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 1-739) protein (GST tag)VCAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-123) protein (GST tag)VCAM1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Cell Adhesion Molecule 1 (VCAM1) (Active) protein (His tag,Fc Tag)VCAM1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-698) protein (His tag)Crystallography grade VCAM1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Vascular Cell Adhesion Molecule 1 (VCAM1) (AA 25-739) protein (rho-1D4 tag)Crystallography grade VCAM1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))
- 别名
- VCAM1 (VCAM1 产品)
- 别名
- CD106 Protein, INCAM-100 Protein, Vcam-1 Protein, VCAM1B Protein, fi76d06 Protein, wu:fi76d06 Protein, zgc:158875 Protein, VCAM1 Protein, vascular cell adhesion molecule 1 Protein, vascular cell adhesion molecule 1b Protein, VCAM1 Protein, Vcam1 Protein, vcam1b Protein, VCAM-1 Protein
- 背景
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Background: Vascular cell adhesion molecule 1 (VCAM-1), also known as CD106, is a cell surface sialoglycoprotein belonging to the immunoglobulin superfamily. Two forms of VCAM-1 with either six or seven extracellular Ig-like domains are generated by alternative splicing, with the longer form predominant. VCAM-1 is an endothelial ligand for very late antigen-4 (VLA-4) and α4ß7 integrin expressed on leukocytes, and thus mediates leukocyte-endothelial cell adhesion and signal transduction. VCAM-1 expression is induced on endothelial cells during inflammatory bowel disease, atherosclerosis, allograft rejection, infection, and asthmatic responses. During these responses, VCAM-1 forms a scaffold for leukocyte migration. VCAM-1 also activates signals within endothelial cells resulting in the opening of an "endothelial cell gate" through which leukocytes migrate. VCAM-1 has been identified as a potential anti-inflammatory therapeutic target, the hypothesis being that reduced expression of VCAM-1 will slow the development of atherosclerosis. In addition, VCAM-1-activated signals in endothelial cells are regulated by cytokines indicating that it is important to consider both endothelial cell adhesion molecule expression and function during inflammatory processes.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: CD106;Vascular cell adhesion protein 1; Vcam1; L1CAM;VCMA1;Vcam-1
- 分子量
- 75.8 kDa
- NCBI登录号
- NP_035823
- 途径
- Carbohydrate Homeostasis
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