CD74 Protein (AA 73-232) (His tag)
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- 抗原 See all CD74 蛋白
- CD74 (HLA-DR-gamma (CD74))
- 蛋白类型
- Recombinant
- 产品特性
- AA 73-232
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This CD74 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- QQQGRLDKLT VTSQNLQLEN LRMKLPKPPK PVSKMRMATP LLMQALPMGA LPQGPMQNAT KYGNMTEDHV MHLLQNADPL KVYPPLKGSF PENLRHLKNT METIDWKVFE SWMHHWLLFE MSRHSLEQKP TDAPPKESLE LEDPSSGLGV TKQDLGPVPM HHHHHH
- 纯度
- > 85 % by SDS - PAGE.
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
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- Discover our top product CD74 蛋白
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HLA-DR-gamma (CD74) (AA 73-232) protein (His tag)
CD74 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
HLA-DR-gamma (CD74) (AA 56-279) protein (His tag)CD74 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
HLA-DR-gamma (CD74) (AA 1-160) protein (GST tag)CD74 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
HLA-DR-gamma (CD74) protein (His tag,mFc Tag)CD74 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
HLA-DR-gamma (CD74) (Active) protein (His tag)CD74 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >90 % as determined by reducing SDS-PAGE. Active
HLA-DR-gamma (CD74) (AA 73-232) protein (His tag)CD74 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In phosphate buffered saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- CD74 (HLA-DR-gamma (CD74))
- 别名
- CD74 (CD74 产品)
- 别名
- DHLAG Protein, HLADG Protein, II Protein, Ia-GAMMA Protein, CLIP Protein, Ii Protein, INVG34 Protein, Iclp-1 Protein, iclp1 Protein, wu:fb11f09 Protein, wu:fb12a01 Protein, wu:fb13d06 Protein, wu:fk94e07 Protein, Iclp-2 Protein, cd74 Protein, iclp2 Protein, zgc:123161 Protein, CD74 molecule Protein, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated) Protein, CD74 molecule, major histocompatibility complex, class II invariant chain a Protein, CD74 molecule, major histocompatibility complex, class II invariant chain b Protein, CD74 Protein, Cd74 Protein, cd74a Protein, cd74b Protein
- 背景
- CD74, also known as HLA class II histocompatibility antigen gamma chain or HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74). The nascent MHC class II protein in the rough ER has its peptide-binding cleft blocked by the invariant chain (Ii, a trimer) to prevent it from binding cellular peptides or peptides from the endogenous pathway. The invariant chain also facilitates MHC class II's export from the ER in a vesicle. The signal for endosomal targeting resides in the cytoplasmic tail of the invariant chain. This fuses with a lateendosome containing the endocytosed, degraded proteins. It is then cleaved by cathepsin S (cathepsin L in cortical thymic epithelial cells), leaving only a small fragment calle CLIP which blocks peptide binding until HLA-DM binds to MHC II, releasing CLIP and allowing other peptides to bind. The stable MHC class-II is then presented on the cell surface. Recombinant human CD74, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 19.05kDa (166aa) 18-28kDa (SDS-PAGE under reducing conditions.)
- NCBI登录号
- NP_004346
- UniProt
- P04233
- 途径
- Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints
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