HLA-DPA1 Protein (AA 29-222, Extracellular) (His tag)
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- 抗原 See all HLA-DPA1 蛋白
- HLA-DPA1 (Major Histocompatibility Complex, Class II, DP alpha 1 (HLA-DPA1))
- 蛋白类型
- Recombinant
- 产品特性
- Extracellular, AA 29-222
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This HLA-DPA1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- AGAIKADHVS TYAAFVQTHR PTGEFMFEFD EDEMFYVDLD KKETVWHLEE FGQAFSFEAQ GGLANIAILN NNLNTLIQRS NHTQATNDPP EVTVFPKEPV ELGQPNTLIC HIDKFFPPVL NVTWLCNGEL VTEGVAESLF LPRTDYSFHK FHYLTFVPSA EDFYDCRVEH WGLDQPLLKH WEAQEPIQMP ETTE
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product HLA-DPA1 蛋白
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Major Histocompatibility Complex, Class II, DP alpha 1 (HLA-DPA1) (AA 1-260) protein (GST tag)
HLA-DPA1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Major Histocompatibility Complex, Class II, DP alpha 1 (HLA-DPA1) (AA 29-222) protein (His tag)Crystallography grade HLA-DPA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Major Histocompatibility Complex, Class II, DP alpha 1 (HLA-DPA1) (AA 29-260) protein (rho-1D4 tag)Crystallography grade HLA-DPA1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- HLA-DPA1 (Major Histocompatibility Complex, Class II, DP alpha 1 (HLA-DPA1))
- 别名
- DPA1 (HLA-DPA1 产品)
- 别名
- DP(W3) Protein, DP(W4) Protein, HLA-DP1A Protein, HLADP Protein, HLASB Protein, PLT1 Protein, major histocompatibility complex, class II, DP alpha 1 Protein, HLA-DPA1 Protein
- 背景
- Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents th on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As mbrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal syst where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The roval of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell mbrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
- 分子量
- 26.4 kDa
- UniProt
- P20036
- 途径
- TCR Signaling, Cancer Immune Checkpoints
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