PHD1 Protein (AA 283-407, partial) (His tag)
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- 抗原 See all PHD1 (EGLN2) 蛋白
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 283-407, partial
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This PHD1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- MVACYPGNGL GYVRHVDNPH GDGRCITCIY YLNQNWDVKV HGGLLQIFPE GRPVVANIEP LFDRLLIFWS DRRNPHEVKP AYATRYAITV WYFDAKERAA AKDKYQLASG QKGVQVPVSQ PPTPT
- 纯化方法
- SDS-PAGE
- 纯度
- > 90 %
- Top Product
- Discover our top product EGLN2 蛋白
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Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-407) protein (GST tag)
EGLN2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-407) protein (Strep Tag)Crystallography grade EGLN2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (AA 1-419) protein (Strep Tag)Crystallography grade EGLN2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)EGLN2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2) protein (His tag)EGLN2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1-2 mg/mL
- 缓冲液
- 20 mM Tris-HCl based buffer, pH 8.0
- 储存条件
- -80 °C,4 °C,-20 °C
- 储存方法
- Store at -20°C, for extended storage, conserve at -20°C or -80°C. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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- 抗原
- PHD1 (EGLN2) (Egl-9 Family Hypoxia Inducible Factor 2 (EGLN2))
- 别名
- EGLN2 (EGLN2 产品)
- 别名
- egln2 Protein, phd2 Protein, PHD1 Protein, 0610011A13Rik Protein, C85656 Protein, Hif-p4h-1 Protein, Ier4 Protein, Phd1 Protein, SM-20 Protein, HIF-PH1 Protein, HPH-1 Protein, HPH-3 Protein, PHD-1 Protein, EIT6 Protein, HIFPH1 Protein, egl-9 family hypoxia-inducible factor 1 Protein, egl-9 family hypoxia inducible factor 2 Protein, Egl nine homolog 2 Protein, egl-9 family hypoxia-inducible factor 2 L homeolog Protein, egl-9 family hypoxia-inducible factor 2 Protein, egln1 Protein, EGLN2 Protein, egln2 Protein, egln2.L Protein, Egln2 Protein
- 背景
- Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF2A. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN2 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle. Also regulates susceptibility to normoxic oxidative neuronal death. Links oxygen sensing to cell cycle and primary cilia formation by hydroxylating the critical centrosome component CEP192 which promotes its ubiquitination and subsequent proteasomal degradation. Hydroxylates IKBKB, mediating NF-kappaB activation in hypoxic conditions. Target proteins are preferencially recognized via a LXXLAP motif.
- 分子量
- 18.2 kDa
- UniProt
- Q96KS0
- 途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Cell RedoxHomeostasis
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