LBP Protein (His tag)
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- 抗原 See all LBP 蛋白
- LBP (Lipopolysaccharide Binding Protein (LBP))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- CHO Cells
- 标记
- This LBP protein is labelled with His tag.
- 应用范围
- ELISA, Agonist (Agon), Flow Cytometry (FACS)
- 序列
- MKAGTGPLLS TLLGLLFLSI QGTGGVNPGV VARITDKGLA YAAKEGLVAL QRELYKITLP DFSGDFKIKA VGRGQYEFHS LEIQNCELRG SSLKLLPGQG LSLAISDSSI GVRGKWKVRK SFLKLHGSFD LDVKGVTISV DLLLGMDPSG RPTVSASGCS SRICDLDVHI SGNVGWLLNL FHNQIESKLQ KVLENKVCEM IQKSVTSDLQ PYLQTLPVTA EIDNVLGIDY SLVAAPQAKA QVLDVMFKGE IFNRNHRSPV FSITDDMLPH DSGIRLNTKA FRPFTPQIYK KYPDMKLELL GTVVSAPILN ATPTPTMSLP EDSKQMVYFA ISDYAFNIAS RVYHQAGYLN VSPGNLSLAP QMEIEGFVIL PTSAREPVFR LGVVTNVFAS LTFNNSKVTG MLHPDKAQVR LIESKVGMFN VNLFQAFLNY YLLNSLYPDV NAELAQGFPL PLPRHIQLHD LDFQIRKDFL YLGANVQYMR VHHHHHH
- 产品特性
- Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off
- 纯化方法
- Affinity chromatography by His-Tag
- 纯度
- >95 % by SDS-PAGE
- 内毒素水平
- <1 EU/μg of recombinant mouse LBP as determined by LAL method
- Biological Activity Comment
- up to 2 μg/ ml LBP mediates binding of FITC-LPS (0.5μg/ml) to CD14+CHO transfectants (2 x 106/ml)
- Top Product
- Discover our top product LBP 蛋白
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Lipopolysaccharide Binding Protein (LBP) (AA 26-477) protein (GST tag)
LBP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lipopolysaccharide Binding Protein (LBP) (AA 26-481) protein (His tag)Crystallography grade LBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL
- 浓度
- 0.25 mg/mL
- 缓冲液
- sterile-filtered phosphate balanced salt solution, pH 7.6
- 储存液
- Without preservative
- 储存条件
- RT,-20 °C,-80 °C
- 储存方法
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Storage: -20°C or -70°C
Storage/Stability: The lyophilized product thought stable at room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Use reconstituted protein immediately or stored in undiluted working aliquots at -20oC. For long-term storage, we recommend add of carrier protein (10% BSA). Repeated freezing and thawing should be avoided!!!The LBP will be precipitate by rapid changes of pH or temperature. Best working temperature is ice bath
- 有效期
- 12 months
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- 抗原
- LBP (Lipopolysaccharide Binding Protein (LBP))
- 别名
- LBP (LBP 产品)
- 别名
- BPIFD2 Protein, Bpifd2 Protein, Ly88 Protein, LPSBP Protein, LBP Protein, lipopolysaccharide binding protein Protein, lipopolysaccharide-binding protein Protein, LBP Protein, Lbp Protein, LOC100472839 Protein
- 背景
- Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes.Recombinant LBP produced from mouse LBP transfected CHO-cells in serum free medium. For transfection, we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection, the complete mouse LBP cDNA amplified by PCR and cloned into expression vector p-POL-DHFR.Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Sequence similarities belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family.
- 分子量
- ~58kDa
- 基因ID
- 16803
- UniProt
- Q61805
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process
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