LBP Protein (His tag)
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- 抗原 See all LBP 蛋白
- LBP (Lipopolysaccharide Binding Protein (LBP))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- CHO Cells
- 标记
- This LBP protein is labelled with His tag.
- 应用范围
- ELISA, Agonist (Agon)
- 序列
- MGALARALPS ILLALLLTST PEALGANPGL VARITDKGLQ YAAQEGLLAL QSELLRITLP DFTGDLRIPH VGRGRYEFHS LNIHSCELLH SALRPVPGQG LSLSISDSSI RVQGRWKVRK SFFKLQGSFD VSVKGISISV NLLLGSESSG RPTVTASSCS SDIADVEVDM SGDLGWLLNL FHNQIESKFQ KVLESRICEM IQKSVSSDLQ PYLQTLPVTT EIDSFADIDY SLVEAPRATA QMLEVMFKGE IFHRNHRSPV FSPGNLSVDP YMEIDAFVLL PSSSKEPVFR LSVATNVSAT LTFNTSKITG FSITDDMIPP DSNIRLTTKS FRPFVPRLAR LYPNMNLELQ GSVPSAPLLN TLLAAVMSLP EEHNKMVYFA ISDYVFNTAS LVYHEEGYLN FLKPGKVKVE LKESKVGLFN AELLEALLNY YILNTFYPKF NDKLAEGFPL PLLKRVQLYD LGLQIHKDFL FLGANVQYMR VHHHHHH
- 产品特性
- Attention: His-tag at the c-terminal end of the LBP has no protease site and is not to split off
- 纯化方法
- Affinity chromatography by His-Tag
- 纯度
- >95 % by SDS-PAGE
- 内毒素水平
- <1 EU/μgof recombinant human LBP as determined by LAL method
- Biological Activity Comment
- Don't activate cells in MNC culture.
- Top Product
- Discover our top product LBP 蛋白
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Lipopolysaccharide Binding Protein (LBP) (AA 26-477) protein (GST tag)
LBP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lipopolysaccharide Binding Protein (LBP) (AA 26-481) protein (His tag)Crystallography grade LBP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 40 μL sterile distilled water to a concentration of 0.25 mg/mL
- 浓度
- 0.25 mg/mL
- 缓冲液
- sterile-filtered phosphate balanced salt solution, pH 7.4
- 储存液
- Without preservative
- 储存条件
- RT,-20 °C,-80 °C
- 储存方法
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Storage: -20°C or -70°C
Storage/Stability: Long time -80oC. Short time storage is possible at 40C or -20C .The lyophilized product thought stable room temperature up to 3 weeks, but is best stored desiccated at -70oC more than 1 year, at refrigerator about 10 months. Reconstituted protein should be used immediately or stored in undiluted working aliquots at -20oC. For long term storage it is recommended to add a carrier protein (o.1% BSA). Repeated freezing and thawing should be avoided!!!
- 有效期
- 12 months
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- 抗原
- LBP (Lipopolysaccharide Binding Protein (LBP))
- 别名
- LBP (LBP 产品)
- 别名
- BPIFD2 Protein, Bpifd2 Protein, Ly88 Protein, LPSBP Protein, LBP Protein, lipopolysaccharide binding protein Protein, lipopolysaccharide-binding protein Protein, LBP Protein, Lbp Protein, LOC100472839 Protein
- 背景
- Background: Natural LBP is a 58KD glycoprotein produced in liver. It binds at lipid A of LPS with high affinity (10-9M) and reduced the cellular LPS effects at CD14+ cells (IL1ß, IL6, TNFα). It acts as opsonin for GRAM negative cells, LPS, neutrophiles and granulocytes. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. Recombinant LBP produced from mouse LBP transfected CHO-cells in serum free medium. For transfection, we have cloned complete mouse LBP cDNA into expression vector pPOL-DHFR. Before transfection, the complete mouse LBP cDNA amplified by PCR and cloned into expression vector p-POL-DHFR. Sequence similarities belongs to the BPI/LBP/Plunc superfamily and BPI/LBP family.
- 分子量
- ~58kDa
- 基因ID
- 3929
- NCBI登录号
- NP_004130
- UniProt
- P18428
- 途径
- TLR signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process
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