Quick Overview for ERK2 蛋白 (ABIN5624604)
抗原
See all ERK2 (MAPK1) 蛋白蛋白类型
宿主
资源
应用范围
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Supplier Product No.
- 009-001-gq9
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Supplier
- Rockland
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原理
- ERK2 human recombinant protein
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产品特性
- MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, ERK-2 recombinant protein
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纯化方法
- ERK2 is a recombinant protein containing a polyhistidine tag expressed in Sf9. Analysis by SDS-PAGE resulted in a pattern consistent with purified ERK2 and was estimated to be greater than 90% pure.
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过滤
- Sterile filtered
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Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 1-360) protein (GST tag)
MAPK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 1-360) protein (His-IF2DI Tag)MAPK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Mitogen-Activated Protein Kinase 1 (MAPK1) protein (GST tag,His tag)MAPK1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 96 % as determined by SDS-PAGE
Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 1-360) protein (His tag)MAPK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
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应用备注
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Application Note: Human ERK2 recombinant protein has been tested in SDS-Page and is suitable as a control for polyclonal or monoclonal anti-ERK2 in immunological assays. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
Other: Dot Blot
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
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有效期
- 12 months
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- ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))
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别名
- MAPK1
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背景
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Synonyms: MAPK1, ERK, ERK-2, ERK2, MAPK2, P42MAPK, PRKM1, PRKM2, ERK-2 recombinant protein
Background: ERK2 recombinant protein was produced in Sf9 cells. Mitogen activated protein kinase 1, also known as MAPK1, ERK, or ERK2, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. ERK1 and ERK2 are activated by MEK1 and MEK2 in the B-raf signaling pathway resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Human ERK1 and ERK2 are 84 % identical in sequence and share common functionality in cells. Recombinant ERK2 protein is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.
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UniProt
- P28482
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途径
- MAPK Pathway, RTK signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins
抗原
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