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- 抗原 See all AKT2 蛋白
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB)
- Supplier Product No.
- 009-001-e71
- Supplier
- Rockland
- 原理
- AKT2 (phosphatase treated) Human Recombinant Protein
- 纯化方法
- Recombinant protein corresponds to amino acids 1 to 479 of mature human AKT2, Akt isoform 2. The recombinant protein contains a polyhistidine affinity tag at the amino terminus. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC.
- Top Product
- Discover our top product AKT2 蛋白
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V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-481) protein (His tag)
custom-made AKT2 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-481) protein (His tag)custom-made AKT2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) proteinAKT2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, WB
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (AA 1-123) protein (His-IF2DI Tag)AKT2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) protein (GST tag,His tag)AKT2 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2) (Active) protein (Myc-DYKDDDDK Tag)AKT2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, PI, STD Active
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- 应用备注
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Application Note: Human AKT2 (phosphatase treated) recombinant protein has been tested by SDS-page and western blot and is suitable as a control for polyclonal or monoclonal anti-AKT2 in immunological assays. Akt2 recombinant protein is dephosphorylated and in an inactive state. It is well suited as a control for anti-AKT pT308 which detects phosphorylated T308 residue, and anti-AKT pS473, that detects phosphorylated S473. For western blot use at 50 ng or less. For other assays concentration is user optimized.
Western Blot Dilution: 50 ng
Other: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.17 mg/mL
- 缓冲液
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Buffer: 20 mM Tris pH 8, 300 mM NaCl with 10 % glycerol
Stabilizer: 10 % (v/v) Glycerol
- 储存液
- Without preservative
- 储存条件
- -20 °C,-80 °C
- 储存方法
- Store vial at -70° C prior to use. Thaw only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. For long term storage we recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 12 months
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- 抗原
- AKT2 (V-Akt Murine Thymoma Viral Oncogene Homolog 2 (AKT2))
- 别名
- AKT2 (AKT2 产品)
- 背景
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Synonyms: RAC, PKB, AKT,PKB beta
Background: AKT2 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and RAC (Related to A and C kinases). AKT is a key regulator of many signal transduction pathways, and it exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. AKT2 recombinant protein is ideal for investigators involved in Cell Signaling, Neuroscience and Signal Transduction research.
- 基因ID
- 208
- UniProt
- P31751
- 途径
- PI3K-Akt Signaling, RTK signaling, AMPK Signaling, TLR signaling, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Hepatitis C, VEGF Signaling
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