IL23 蛋白
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- 抗原 See all IL23 蛋白
- IL23 (Interleukin 23 (IL23))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Insect cells (Sf9)
- 应用范围
- Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
- 原理
- Purified recombinant protein of Human interleukin 23 (p19+p40).
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
- Human IL-23 consists of two subunits linked via a disulfide bond: P19, the region of Ala21- Pro189) from Accession# NP_057668, and P40, the region of Ile 23-Ser 328) from Accession# NP_002178.2
- 纯度
- > 95 % , as determined by Coomassie stained SDS-PAGE.
- 内毒素水平
- Less than 0.01 ng per μg protein as determined by the LAL method.
- Biological Activity Comment
- The ED50 is 0.4 - 2.0 ng/ml, corresponding to a specific activity of 0.5 - 2.50 x 10^6 units/mg determined by mouse splenocyte IL-17A secretion, which is induced by hIL-23 in a dose dependent manner.
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- Discover our top product IL23 蛋白
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Interleukin 23 (IL23) protein
IL23 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 23 (IL23) proteinIL23 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 23 (IL23) protein (His tag)IL23 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 23 (IL23) protein (Fc Tag)IL23 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 23 (IL23) (Active) protein (His tag)IL23 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 浓度
- > 50 μg/mL
- 缓冲液
- 1 x PBS
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- IL23 (Interleukin 23 (IL23))
- 别名
- IL23 (IL23 产品)
- 别名
- IL-23 Protein, p19 Protein, SGRF Protein, il23p19 Protein, interleukin 23, alpha subunit p19 Protein, interleukin 23 subunit alpha Protein, Il23a Protein, IL23A Protein
- 背景
- This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.
- 分子量
- 53.3 kDa
- NCBI登录号
- NP_057668
- UniProt
- Q9NPF7
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