IL23 Protein (His tag)
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- 抗原 See all IL23 蛋白
- IL23 (Interleukin 23 (IL23))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This IL23 protein is labelled with His tag.
- 原理
- Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)(Active)
- 序列
- Met 1-Pro189&Met 1-Ser 28
- 产品特性
- A DNA sequence encoding the IL23A (Q9NPF7) (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1, A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- 1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.2. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml.3. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml.4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL.
- Top Product
- Discover our top product IL23 蛋白
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Interleukin 23 (IL23) protein
IL23 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Interleukin 23 (IL23) proteinIL23 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Interleukin 23 (IL23) protein (His tag)IL23 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 23 (IL23) protein (Fc Tag)IL23 宿主: 人 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- IL23 (Interleukin 23 (IL23))
- 别名
- IL-23 (IL23 产品)
- 别名
- IL-23 Protein, p19 Protein, SGRF Protein, il23p19 Protein, interleukin 23, alpha subunit p19 Protein, interleukin 23 subunit alpha Protein, Il23a Protein, IL23A Protein
- 背景
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Background: Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
Synonym: SGRF,IL-23p19,CLMF p40,IL-12 subunit p40,NKSF2,IL-23,IL-23A,IL23P19,P19
- 分子量
- 56.3 (20.1 + 36.2) kDa
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