IGFBP3 Protein (Transcript Variant 2)
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- 抗原 See all IGFBP3 蛋白
- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- Transcript Variant 2
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Functional Studies (Func), Antibody Production (AbP), Protein Interaction (PI), Standard (STD)
- 特异性
- Optimal preservation of protein structure, post-translational modifications and functions.
- 产品特性
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- Recombinant human IGFBP3 (transcript variant 2) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
- 纯度
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- 内毒素水平
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
- Biological Activity Comment
- ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 for this effect is less than or equal to 0.2 ug/ml in presence of 15 ng/ml of human IGF-II.
- Top Product
- Discover our top product IGFBP3 蛋白
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Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (Active) protein
IGFBP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 28-291) protein (His tag)IGFBP3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 120-244) protein (His tag)IGFBP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 28-291) protein (His tag)IGFBP3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Insulin-Like Growth Factor Binding Protein 3 (IGFBP3) (AA 182-291) protein (GST tag)IGFBP3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays - 限制
- 仅限研究用
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- 缓冲液
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
- 注意事项
- Resuspend the protein in the desired concentration in proper buffer
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- 抗原
- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
- 别名
- IGFBP3 (IGFBP3 产品)
- 别名
- BP-53 Protein, IBP3 Protein, AI649005 Protein, IGFBP-3 Protein, IGgfbp3 Protein, IGF-BP3 Protein, zgc:91788 Protein, IGFBP3 Protein, igfbp3 Protein, insulin like growth factor binding protein 3 Protein, insulin-like growth factor binding protein 3 Protein, IGFBP3 Protein, Igfbp3 Protein, igfbp3 Protein, IGFBP-3 Protein, LOC100305016 Protein
- 背景
- This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein forms a ternary complex with insulin-like growth factor acid-labile subunit (IGFALS) and either insulin-like growth factor (IGF) I or II. In this form, it circulates in the plasma, prolonging the half-life of IGFs and altering their interaction with cell surface receptors. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
- 分子量
- 28.8 kDa
- NCBI登录号
- NP_000589
- 途径
- Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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