SLAMF7 Protein (AA 23-333) (rho-1D4 tag)
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- 抗原 See all SLAMF7 蛋白
- SLAMF7 (SLAM Family Member 7 (SLAMF7))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-333
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This SLAMF7 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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SGTLKKVAGA LDGSVTFTLN ITEIKVDYVV WTFNTFFLAM VKKDGVTSQS SNKERIVFPD GLYSMKLSQL KKNDSGAYRA EIYSTSSQAS LIQEYVLHVY KHLSRPKVTI DRQSNKNGTC VINLTCSTDQ DGENVTYSWK AVGQGDNQFH DGATLSIAWR SGEKDQALTC MARNPVSNSF STPVFPQKLC EDAATDLTSL RGILYILCFS AVLILFAVLL TIFHTTWIKK GKGCEEDKKR VDRHQEMPDL CPHLEENADY DTIPYTEKRR PEEDAPNTFY STVQIPKVVK SPSSLPAKPL VPRSLSFENV I
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Slamf7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SLAMF7 蛋白
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SLAM Family Member 7 (SLAMF7) (AA 23-226) protein (Fc Tag)
SLAMF7 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SLAM Family Member 7 (SLAMF7) (AA 23-226) protein (His-Avi Tag)SLAMF7 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SLAM Family Member 7 (SLAMF7) (AA 23-226) protein (mFc Tag)SLAMF7 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SLAM Family Member 7 (SLAMF7) (AA 23-226) protein (His-Avi Tag,Biotin)SLAMF7 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SLAM Family Member 7 (SLAMF7) (AA 23-224) protein (His tag)SLAMF7 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SLAM Family Member 7 (SLAMF7) (AA 23-226) protein (His tag)SLAMF7 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SLAM Family Member 7 (SLAMF7) (AA 23-226) protein (His tag)SLAMF7 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
SLAM Family Member 7 (SLAMF7) (AA 23-226) protein (His tag,AVI tag,Biotin)SLAMF7 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE.
SLAM Family Member 7 (SLAMF7) (AA 23-224) protein (His tag)SLAMF7 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
SLAM Family Member 7 (SLAMF7) protein (His tag)SLAMF7 宿主: 猕猴 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE.
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SLAMF7 (SLAM Family Member 7 (SLAMF7))
- 别名
- Slamf7 (SLAMF7 产品)
- 别名
- RGD1560190 Protein, SLAMF7 Protein, slaf7 Protein, 19A Protein, CD319 Protein, CRACC Protein, CS1 Protein, 19A24 Protein, 4930560D03Rik Protein, SLAM family member 7 Protein, Slamf7 Protein, SLAMF7 Protein, slaf7 Protein
- 背景
- Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:19648922). Mediates natural killer (NK) cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (By similarity). Positively regulates NK cell functions by a mechanism dependent on the adapter SH2D1B. In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (PubMed:19151721). May play a role in lymphocyte adhesion (By similarity). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (By similarity). {ECO:0000250|UniProtKB:Q9NQ25, ECO:0000269|PubMed:19151721, ECO:0000269|PubMed:19648922}.
- 分子量
- 35.9 kDa Including tag.
- UniProt
- Q8BHK6
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