Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-1138) protein (Strep Tag)
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- 抗原 See all Chromosome 11open Reading Frame 9 (C11orf9) 蛋白
- Chromosome 11open Reading Frame 9 (C11orf9)
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-1138
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- Strep Tag
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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MEVVDETEAL QRFFEGHDIS GALEPSNIDT SILEEYIGKE DASDLCFPEI SAPASTASFP HGPPAIPGSS GLHHLSPPGS GPSPGRHGPL PPPTYGTPLN CNNNNGMGTA PKPFLGGSGP PIKAEPKAPY APGTLPDSPP DSGSEAYSPQ QVNDPHLLRT ITPETLCHVG VSSRLEHPPP PPAHLPGPPP PPPPPPHYPV LQRDLYMKAE PPVPPYAAMG PGLVPPELHH TQQTQVLHQL LQQHGAELPP HPSKKRKHSE SPPNTLNAQM LNGMIKQEPG TVTALPPHPA RAPSPPWPPQ GPLSPGTGSL PLSIARAQTP PWHPPGAPSP GLLQDSDSLS GSYLDPNYQS IKWQPHQQNK WATLYDANYK ELPMLTYRVD ADKGFNFSVG DDAFVCQKKN HFQVTVYIGM LGEPKYVKTP EGLKPLDCFY LKLHGVKLEA LNQSINIEQS QSDRSKRPFN PVTVNLPPEQ VTKVTVGRLH FSETTANNMR KKGKPNPDQR YFMLVVALQA HAQNQNYTLA AQISERIIVR ASNPGQFESD SDVLWQRAQL PDTVFHHGRV GINTDRPDEA LVVHGNVKVM GSLMHPSDLR AKEHVQEVDT TEQLKRISRM RLVHYRYKPE FAASAGIEAT APETGVIAQE VKEILPEAVK DTGDVVFANG KTIENFLVVN KERIFMENVG AVKELCKLTD NLETRIDELE RWSHKLAKLR RLDSLKSTGS SGAFSHAGSQ FSRAGSVPHK KRPPKLANKS SPAVPDQACI SQRFLQGTII ALVVVMAFSV VSMSTLYVLS LRSEEDLVDA DGSLAVSTSC LLALLRPQDP GGSEAMCPWS SQSFGTTQLR QSSMTTGLPG TQPSLLLVTK SASGPALRAL DLCSSQPCPI VCCSPPVSSP ATDPALGPTL TPTPSPSSNP KHSGPGQMAP LPVTNIRAKS WGISANGISY SKHSKSLEPL ASPVVPFPGG QSKTKNSPSF NLQSRARRGA PQPSPSPAQF TQTQGQLDPA PSLTSIQLLE NSMPITSQYC VPEGACRLGN FTYHIPVSSS TPLHLSLTLQ MNSSTPVSVV LCSLTSEEEP CEEGGFLQRF HPHQDTQGTS HQWPVTILSF REFTYHFRVT LLGQANCSSE AIVQPATDYY FHFYRLCD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product C11orf9 蛋白
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Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-1151) protein (Strep Tag)
Crystallography grade C11orf9 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Chromosome 11open Reading Frame 9 (C11orf9) (AA 790-1151) protein (His tag)Crystallography grade C11orf9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 790-1138) protein (His tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-586) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 587-1151) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-586) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 587-1138) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-768) protein (His tag)Crystallography grade C11orf9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-768) protein (His tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Chromosome 11open Reading Frame 9 (C11orf9)
- 别名
- Myrf (C11orf9 产品)
- 别名
- C11orf9 Protein, c11orf9 Protein, mrf Protein, ndt80 Protein, MRF Protein, Ndt80 Protein, pqn-47 Protein, 6030439E18 Protein, Gm1804 Protein, Gm98 Protein, Mrf Protein, chromosome 5 open reading frame, human C11orf9 Protein, myelin regulatory factor L homeolog Protein, myelin regulatory factor Protein, C5H11ORF9 Protein, myrf.L Protein, MYRF Protein, Myrf Protein
- 背景
- Myelin regulatory factor (EC 3.4.-.-) (Myelin gene regulatory factor) [Cleaved into: Myelin regulatory factor, N-terminal, Myelin regulatory factor, C-terminal],FUNCTION: [Myelin regulatory factor]: Constitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes. {ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:28623291}., FUNCTION: [Myelin regulatory factor, C-terminal]: Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage. {ECO:0000269|PubMed:23966833}., FUNCTION: [Myelin regulatory factor, N-terminal]: Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG, DUSP15 and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination (PubMed:19596243, PubMed:22956843, PubMed:23966833, PubMed:24204311, PubMed:27532821). Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype (PubMed:19596243, PubMed:22956843, PubMed:23966833). {ECO:0000269|PubMed:19596243, ECO:0000269|PubMed:22956843, ECO:0000269|PubMed:23966833, ECO:0000269|PubMed:24204311, ECO:0000269|PubMed:27532821}.
- 分子量
- 123.3 kDa
- UniProt
- Q3UR85
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