Chromosome 11open Reading Frame 9 (C11orf9) (AA 790-1151) protein (His tag)
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- 抗原 See all Chromosome 11open Reading Frame 9 (C11orf9) 蛋白
- Chromosome 11open Reading Frame 9 (C11orf9)
- 蛋白类型
- Recombinant
- 产品特性
- AA 790-1151
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宿主
- 人
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资源
- Insect Cells
- 标记
- His tag
- 应用范围
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- 序列
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SLRTEEDLVD TDGSFAVSTS CLLALLRPQP PGGSEALCPW SSQSFGTTQL RQSPLTTGLP GIQPSLLLVT TSLTSSAPGS AVRTLDMCSS HPCPVICCSS PTTNPTTGPS LGPSFNPGHV LSPSPSPSTN RSGPSQMALL PVTNIRAKSW GLSVNGIGHS KHHKSLEPLA SPAVPFPGGQ GKAKNSPSLG FHGRARRGAL QSSVGPAEPT WAQGQSASLL AEPVPSLTSI QVLENSMSIT SQYCAPGDAC RPGNFTYHIP VSSGTPLHLS LTLQMNSSSP VSVVLCSLRS KEEPCEEGSL PQSLHTHQDT QGTSHRWPIT ILSFREFTYH FRVALLGQAN CSSEALAQPA TDYHFHFYRL CD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MYRF Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
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Chromosome 11open Reading Frame 9 (C11orf9) (AA 790-1138) protein (His tag)
Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-1151) protein (Strep Tag)Crystallography grade C11orf9 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-586) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 587-1151) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-586) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 587-1138) protein (rho-1D4 tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-768) protein (His tag)Crystallography grade C11orf9 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-768) protein (His tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
Chromosome 11open Reading Frame 9 (C11orf9) (AA 1-1138) protein (Strep Tag)Crystallography grade C11orf9 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Chromosome 11open Reading Frame 9 (C11orf9)
- 别名
- MYRF (C11orf9 产品)
- 别名
- C11orf9 Protein, c11orf9 Protein, mrf Protein, ndt80 Protein, MRF Protein, Ndt80 Protein, pqn-47 Protein, 6030439E18 Protein, Gm1804 Protein, Gm98 Protein, Mrf Protein, chromosome 5 open reading frame, human C11orf9 Protein, myelin regulatory factor L homeolog Protein, myelin regulatory factor Protein, C5H11ORF9 Protein, myrf.L Protein, MYRF Protein, Myrf Protein
- 背景
- Myelin regulatory factor: Consitutes a precursor of the transcription factor. Mediates the autocatalytic cleavage that releases the Myelin regulatory factor, N-terminal component that specifically activates transcription of central nervous system (CNS) myelin genes (PubMed:23966832). {ECO:0000269|PubMed:23966832}., Myelin regulatory factor, C-terminal: Membrane-bound part that has no transcription factor activity and remains attached to the endoplasmic reticulum membrane following cleavage. {ECO:0000269|PubMed:23966832}., Myelin regulatory factor, N-terminal: Transcription factor that specifically activates expression of myelin genes such as MBP, MOG, MAG and PLP1 during oligodendrocyte (OL) maturation, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Specifically recognizes and binds DNA sequence 5'-CTGGYAC-3' in the regulatory regions of myelin-specific genes and directly activates their expression. Not only required during oligodendrocyte differentiation but is also required on an ongoing basis for the maintenance of expression of myelin genes and for the maintenance of a mature, viable oligodendrocyte phenotype (PubMed:23966832). {ECO:0000269|PubMed:23966832}.
- 分子量
- 39.2 kDa Including tag.
- UniProt
- Q9Y2G1
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