CD39 Protein (AA 1-510) (Strep Tag)
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- 抗原 See all CD39 (ENTPD1) 蛋白
- CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-510
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This CD39 protein is labelled with Strep Tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- 序列
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MEDIKDSKVK RFCSKNILII LGFTSILAVI ALIAVGLTQN KPLPENVKYG IVLDAGSSHT NLYIYKWPAE KENDTGVVQQ LEECQVKGPG ISKYAQKTDE IGAYLAECME LSTELIPTSK HHQTPVYLGA TAGMRLLRME SEQSADEVLA AVSTSLKSYP FDFQGAKIIT GQEEGAYGWI TINYLLGRFT QEQSWLSLIS DSQKQETFGA LDLGGASTQI TFVPQNSTIE SPENSLQFRL YGEDYTVYTH SFLCYGKDQA LWQKLAKDIQ VSSGGVLKDP CFNPGYEKVV NVSELYGTPC TKRFEKKLPF DQFRIQGTGD YEQCHQSILE LFNNSHCPYS QCAFNGVFLP PLHGSFGAFS AFYFVMDFFK KVAKNSVISQ EKMTEITKNF CSKSWEETKT SYPSVKEKYL SEYCFSGAYI LSLLQGYNFT DSSWEQIHFM GKIKDSNAGW TLGYMLNLTN MIPAEQPLSP PLPHSTYIGL MVLFSLLLVA VAITGLFIYS KPSYFWKEAV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ENTPD1 蛋白
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Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 38-478) protein (His tag)
ENTPD1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 70-197) protein (His-IF2DI Tag)ENTPD1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 1-510) protein (GST tag)ENTPD1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (Active) protein (His tag)ENTPD1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE Active
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 1-510) protein (Strep Tag)Crystallography grade ENTPD1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 38-478) protein (His tag)Crystallography grade ENTPD1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 38-478) protein (His tag)Crystallography grade ENTPD1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 38-478) (Active) protein (His tag)ENTPD1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1) (AA 38-478) (Active) protein (mFc Tag)ENTPD1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))
- 别名
- Entpd1 (ENTPD1 产品)
- 别名
- ATPDase Protein, CD39 Protein, NTPDase-1 Protein, MGC80631 Protein, zgc:100819 Protein, entpd1 Protein, MGC79567 Protein, 2610206B08Rik Protein, AA408691 Protein, Cd39 Protein, ectonucleoside triphosphate diphosphohydrolase 1 Protein, ectonucleoside triphosphate diphosphohydrolase 1 L homeolog Protein, ENTPD1 Protein, Entpd1 Protein, entpd1.L Protein, entpd1 Protein
- 背景
- Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) (EC 3.6.1.5) (ATP diphosphohydrolase) (ATP-DPH) (ATPDase) (Ecto-ATP diphosphohydrolase 1) (Ecto-ATPDase 1) (Ecto-ATPase 1) (Ecto-apyrase) (Lymphoid cell activation antigen) (Nucleoside triphosphate diphosphohydrolase 1) (NTPDase1) (CD antigen CD39),FUNCTION: Catalyzes the hydrolysis of both di- and triphosphate nucleotides (NDPs and NTPs) and hydrolyze NTPs to nucleotide monophosphates (NMPs) in two distinct successive phosphate-releasing steps, with NDPs as intermediates and participates in the regulation of extracellular levels of nucleotides. By hydrolyzing proinflammatory ATP and platelet-activating ADP to AMP, it blocks platelet aggregation and supports blood flow. {ECO:0000250|UniProtKB:P49961}.
- 分子量
- 57.2 kDa
- UniProt
- P55772
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