SLC27A2 Protein (AA 1-620) (Strep Tag)
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- 抗原 See all SLC27A2 蛋白
- SLC27A2 (Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-620
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This SLC27A2 protein is labelled with Strep Tag.
- 应用范围
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- 序列
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MLPVLYTGLA GLLLLPLLLT CCCPYLLQDV RYFLRLANMA RRVRSYRQRR PVRTILRAFL EQARKTPHKP FLLFRDETLT YAQVDRRSNQ VARALHDQLG LRQGDCVALF MGNEPAYVWI WLGLLKLGCP MACLNYNIRA KSLLHCFQCC GAKVLLASPD LQEAVEEVLP TLKKDAVSVF YVSRTSNTNG VDTILDKVDG VSAEPTPESW RSEVTFTTPA VYIYTSGTTG LPKAATINHH RLWYGTGLAM SSGITAQDVI YTTMPLYHSA ALMIGLHGCI VVGATLALRS KFSASQFWDD CRKYNVTVIQ YIGELLRYLC NTPQKPNDRD HKVKKALGNG LRGDVWREFI KRFGDIHVYE FYASTEGNIG FVNYPRKIGA VGRANYLQRK VARYELIKYD VEKDEPVRDA NGYCIKVPKG EVGLLVCKIT QLTPFIGYAG GKTQTEKKKL RDVFKKGDIY FNSGDLLMID RENFVYFHDR VGDTFRWKGE NVATTEVADI VGLVDFVEEV NVYGVPVPGH EGRIGMASLK IKENYEFNGK KLFQHIAEYL PSYARPRFLR IQDTIEITGT FKHRKVTLME EGFNPTVIKD TLYFMDDAEK TFVPMTENIY NAIIDKTLKL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product SLC27A2 蛋白
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Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 28-106) protein (His-IF2DI Tag)
SLC27A2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 25-123) protein (GST tag)SLC27A2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 1-567) protein (GST tag)SLC27A2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 1-620) protein (Strep Tag)Crystallography grade SLC27A2 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 289-620) protein (His tag)Crystallography grade SLC27A2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 128-261) protein (His tag)Crystallography grade SLC27A2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 283-620) protein (His tag)Crystallography grade SLC27A2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 128-267) protein (His tag)Crystallography grade SLC27A2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 283-620), (Cytoplasmic Domain), (Cytosolic) protein (His-SUMO Tag)SLC27A2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2) (AA 283-620), (Cytoplasmic Domain), (Cytosolic) protein (His tag)SLC27A2 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- SLC27A2 (Solute Carrier Family 27 (Fatty Acid Transporter), Member 2 (SLC27A2))
- 别名
- Slc27a2 (SLC27A2 产品)
- 别名
- vlcs Protein, fatp2 Protein, vlacs Protein, acsvl1 Protein, facvl1 Protein, im:7139614 Protein, slc27a2 Protein, wu:fb99g05 Protein, zgc:112376 Protein, ACSVL1 Protein, FACVL1 Protein, HsT17226 Protein, hFACVL1 Protein, FATP2 Protein, VLCS Protein, Vlac Protein, Vlacs Protein, VLACS Protein, solute carrier family 27 (fatty acid transporter), member 2 L homeolog Protein, solute carrier family 27 member 2 Protein, solute carrier family 27 (fatty acid transporter), member 2a Protein, solute carrier family 27 (fatty acid transporter), member 2 Protein, slc27a2.L Protein, SLC27A2 Protein, slc27a2a Protein, slc27a2 Protein, Slc27a2 Protein
- 背景
- Long-chain fatty acid transport protein 2 (Arachidonate--CoA ligase) (EC 6.2.1.15) (Fatty acid transport protein 2) (FATP-2) (Fatty-acid-coenzyme A ligase, very long-chain 1) (Long-chain-fatty-acid--CoA ligase) (EC 6.2.1.3) (Phytanate--CoA ligase) (EC 6.2.1.24) (Solute carrier family 27 member 2) (Slc27a2) (THCA-CoA ligase) (EC 6.2.1.7) (Very long-chain acyl-CoA synthetase) (VLACS) (VLCS) (EC 6.2.1.-) (Very long-chain-fatty-acid-CoA ligase),FUNCTION: Mediates the import of long-chain fatty acids (LCFA) into the cell by facilitating their transport across cell membranes, playing an important role in hepatic fatty acid uptake (PubMed:9671728, PubMed:15699031, PubMed:20530735). Also functions as an acyl-CoA ligase catalyzing the ATP-dependent formation of fatty acyl-CoA using LCFA and very-long-chain fatty acids (VLCFA) as substrates, which prevents fatty acid efflux from cells and might drive more fatty acid uptake (PubMed:15699031, PubMed:20530735). Plays a pivotal role in regulating available LCFA substrates from exogenous sources in tissues undergoing high levels of beta-oxidation or triglyceride synthesis (PubMed:15699031, PubMed:20530735). Can also activate branched-chain fatty acids such as phytanic acid and pristanic acid (By similarity). May contribute to the synthesis of sphingosine-1-phosphate (By similarity). Does not activate C24 bile acids, cholate and chenodeoxycholate (By similarity). In vitro, activates 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanate (THCA), the C27 precursor of cholic acid deriving from the de novo synthesis from cholesterol. However, it is not critical for THCA activation and bile synthesis in vivo (By similarity). {ECO:0000250|UniProtKB:O14975, ECO:0000269|PubMed:15699031, ECO:0000269|PubMed:20530735, ECO:0000269|PubMed:9671728}.
- 分子量
- 70.4 kDa
- UniProt
- O35488
- 途径
- Monocarboxylic Acid Catabolic Process, SARS-CoV-2 Protein Interactome
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