电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

POLI Protein (AA 1-717) (Strep Tag)

Crystallography grade POLI 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
产品编号 ABIN3135830
发货至: 中国
  • 抗原 See all POLI 蛋白
    POLI (Polymerase (DNA Directed) iota (POLI))
    蛋白类型
    Recombinant
    产品特性
    AA 1-717
    宿主
    小鼠
    资源
    • 2
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    标记
    This POLI protein is labelled with Strep Tag.
    应用范围
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    序列
    MEPLHAGAAG SSRAVCSQGP PTQISSSRVI VHVDLDCFYA QVEMISNPEL KDRPLGVQQK YLVVTCNYEA RKLGVRKLMN VRDAKEKCPQ LVLVNGEDLS RYREMSYKVT ELLEEFSPAV ERLGFDENFV DLTEMVEKRL QQLPSEEVPS VTVFGHVYNN QSVNLHNIMH RRLVVGSQIA AEMREAMYNQ LGLTGCAGVA PNKLLAKLVS GVFKPNQQTV LLPESCQHLI HSLNHIKEIP GIGYKTAKRL EVLGINSVHD LQTFPIKTLE KELGIAIAQR IQQLSFGEDK SPVTPSGPPQ SFSEEDTFKK CSSEVEAKAK IEELLSSLLT RVCQDGRKPH TVRLVIRRYS DKHCNRESRQ CPIPSHVIQK LGTGNHDSMP PLIDILMKLF RNMVNVKMPF HLTLMSVCFC NLKALSSAKK GPMDCYLTSL STPAYTDKRA FKVKDTHTED SHKEKEANWD CLPSRRIEST GTGESPLDAT CFPKEKDTSD LPLQALPEGV DQEVFKQLPA DIQEEILYGK SRENLKGKGS LSCPLHASRG VLSFFSTKQM QASRLSPRDT ALPSKRVSAA SPCEPGTSGL SPGSTSHPSC GKDCSYYIDS QLKDEQTSQG PTESQGCQFS STNPAVSGFH SFPNLQTEQL FSTHRTVDSH KQTATASHQG LESHQGLESR ELDSAEEKLP FPPDIDPQVF YELPEEVQKE LMAEWERAGA ARPSAHR
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    产品特性
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    纯化方法
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    纯度
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    内毒素水平
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    质量等级
    Crystallography grade
    Top Product
    Discover our top product POLI 蛋白
  • 应用备注
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    说明

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    注意事项
    Avoid repeated freeze-thaw cycles.
    储存条件
    -80 °C
    储存方法
    Store at -80°C.
    有效期
    Unlimited (if stored properly)
  • 抗原
    POLI (Polymerase (DNA Directed) iota (POLI))
    别名
    Poli (POLI 产品)
    别名
    poli Protein, wu:fc27h11 Protein, zgc:110185 Protein, GB12093 Protein, NV16187 Protein, RAD30B Protein, RAD3OB Protein, Rad30b Protein, DNA polymerase iota Protein, polymerase (DNA directed) iota Protein, polymerase (DNA directed), iota Protein, POLI Protein, poli Protein, LOC551219 Protein, AFUA_4G11140 Protein, LOC5568276 Protein, Pol-iota Protein, Poli Protein
    背景
    DNA polymerase iota (EC 2.7.7.7) (Rad30 homolog B),FUNCTION: Error-prone DNA polymerase specifically involved in DNA repair (PubMed:15026325, PubMed:16357261). Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:15026325, PubMed:16357261). Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template (By similarity). Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine (By similarity). May play a role in hypermutation of immunoglobulin genes (By similarity). Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity (By similarity). {ECO:0000250|UniProtKB:Q9UNA4, ECO:0000269|PubMed:15026325, ECO:0000269|PubMed:16357261}.
    分子量
    79.7 kDa
    UniProt
    Q6R3M4
    途径
    DNA Damage Repair
You are here:
客服