RAGE Protein (AA 23-340) (His tag)
-
- 抗原 See all RAGE (AGER) 蛋白
- RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-340
-
宿主
- 小鼠
-
资源
- Insect Cells
- 标记
- This RAGE protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
GQNITARIGE PLVLSCKGAP KKPPQQLEWK LNTGRTEAWK VLSPQGGPWD SVARILPNGS LLLPATGIVD EGTFRCRATN RRGKEVKSNY RVRVYQIPGK PEIVDPASEL TASVPNKVGT CVSEGSYPAG TLSWHLDGKL LIPDGKETLV KEETRRHPET GLFTLRSELT VIPTQGGTHP TFSCSFSLGL PRRRPLNTAP IQLRVREPGP PEGIQLLVEP EGGIVAPGGT VTLTCAISAQ PPPQVHWIKD GAPLPLAPSP VLLLPEVGHE DEGTYSCVAT HPSHGPQESP PVSIRVTETG DEGPAEGSVG ESGLGTLA
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ager Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product AGER 蛋白
-
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-340) protein (His tag)
AGER 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-344) protein (His tag)AGER 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-342) protein (His tag)AGER 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-342) protein (His-DYKDDDDK-Strep II Tag)AGER 宿主: 人 宿主: HEK-293T Cells Recombinant WB, SDS, ELISA, PI
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 362-404) protein (His-IF2DI Tag)AGER 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 134-231) protein (GST tag)AGER 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 23-404) protein (GST tag)AGER 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 87-197) protein (GST tag)AGER 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Advanced Glycosylation End Product-Specific Receptor (AGER) (AA 1-237) protein (GST tag)AGER 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Advanced Glycosylation End Product-Specific Receptor (AGER) (Active) protein (His tag)AGER 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
- 别名
- Ager (AGER 产品)
- 别名
- RAGE Protein, AGER Protein, advanced glycosylation end-product specific receptor Protein, advanced glycosylation end product-specific receptor Protein, MAPK/MAK/MRK overlapping kinase Protein, AGER Protein, Ager Protein, LOC719012 Protein
- 背景
- Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Can also bind oligonucleotides. Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. RAGE-dependent signaling in microglia contributes to neuroinflammation, amyloid accumulation, and impaired learning/memory in a mouse model of Alzheimer disease. {ECO:0000269|PubMed:10399917, ECO:0000269|PubMed:18539754, ECO:0000269|PubMed:19901339, ECO:0000269|PubMed:19906677}., Isoform 2: Is able to advanced glycosylation end product (AGE)-induce nuclear factor NF-kappa-B activation. {ECO:0000269|PubMed:16503878}., Isoform 10: Down-regulates receptor for advanced glycosylation end products (RAGE)-ligand induced signaling through various MAPK pathways including ERK1/2, p38 and SAPK/JNK. Significantly affects tumor cell properties through decreasing cell migration, invasion, adhesion and proliferation, and increasing cellular apoptosis. Exhibits drastic inhibition on tumorigenesis in vitro. {ECO:0000269|PubMed:24260107}.
- 分子量
- 34.7 kDa Including tag.
- UniProt
- Q62151
- 途径
- Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
-
