GSAP Protein (AA 1-858) (Strep Tag)
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- 抗原 See all GSAP (PION) 蛋白
- GSAP (PION) (Pigeon Homolog (PION))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-858
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This GSAP protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MALRLVTHFD VLEDVLPSLL TQAATTDEGD RAGVLETTYG SLRVLNIERN GNIIYTYKDN KGNAVFGLYD CQTRQNEHLY TFEKDMQAVS CSVNSERTVL AASFIQYTTE GVKNDLQPGS KCLTLLVEIH PVNNVKVLKA VDSCVWVQFL YPQAESHLLP QNHLLLISEE KYIERFHIQI TREDGDRVVI RNSSHLPRDR LAEDFVWAQW DLSEQRLYYI ELKESRSILK CIQFRADESF NLMFEMPLDI TLTGLRFKLV NFGYDYRQDR EKLCNQPSLC IFTNHTGSLC MCYSPKSDSR EEITYSVFYL HKGYRKIFTA APGSADSQVT NGADSQVTDG IAFLNLGYFV AVYSPGHFLH LLNIQHPDLV CHSLFLTGNN KIAAVLPPSP LQSLPGSLVL DCYSGKVYRV TLDQSYLLRF LWNAHLDCER MAALHCILSC SQDPGFPEEQ IIQWISEHVS ACHSFDLIQE FLIASSYWSV YAELDDMGML LQYSSVLTWN TEIPGIKFTT EELPLPLMKV YGLKGYWAKL NSNLEYIKYT KPHLHYHNSV VRREWHNLIS EERTGKRRST MYVRNILENA MKVIASMETR TLEPRLIPFL QEEDRHQRLL MGLMVSELRD HLLRHLQGVE KKKIEQMVLD YISKLLDLIW CLLETSWRKH SMHPLVLHLN SHCSAADFEV FHLMTRILDA ASSLCLPLPP GFHSLHTILG VHCLPLYSLL HYIDNGVLLL TETAVTRLMK DLDNSEKNEQ LKFSIIVRLP PLIGQKVCRL WDHPMSSNII SRNHVARLLK NYRKEPRNSM IDKSSFPVEF LPLNYFIEIL MGLESSNQAL YGFEGHDNVD AEFVEEAALK HTTMLLGL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product PION 蛋白
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Pigeon Homolog (PION) (AA 1-854) protein (Strep Tag)
Crystallography grade PION 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Pigeon Homolog (PION) (AA 734-854) protein (His tag)Crystallography grade PION 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pigeon Homolog (PION) (AA 738-858) protein (His tag)Crystallography grade PION 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Pigeon Homolog (PION) proteinPION 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- GSAP (PION) (Pigeon Homolog (PION))
- 别名
- Gsap (PION 产品)
- 别名
- PION Protein, A530088I07Rik Protein, Pion Protein, gamma-secretase activating protein Protein, GSAP Protein, Gsap Protein
- 背景
- Gamma-secretase-activating protein (GSAP) (Protein pigeon homolog) [Cleaved into: Gamma-secretase-activating protein 16 kDa C-terminal form (GSAP-16K)],FUNCTION: Regulator of gamma-secretase activity, which specifically activates the production of amyloid-beta protein (amyloid-beta protein 40 and amyloid-beta protein 42), without affecting the cleavage of other gamma-secretase targets such has Notch. The gamma-secretase complex is an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Specifically promotes the gamma-cleavage of APP CTF-alpha (also named APP-CTF) by the gamma-secretase complex to generate amyloid-beta, while it reduces the epsilon-cleavage of APP CTF-alpha, leading to a low production of AICD. {ECO:0000269|PubMed:20811458}.
- 分子量
- 98.7 kDa
- UniProt
- Q3TCV3
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