HNRNPA1 Protein (AA 1-320) (Strep Tag)
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- 抗原 See all HNRNPA1 蛋白
- HNRNPA1 (Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-320
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宿主
- 小鼠
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资源
- Tobacco (Nicotiana tabacum)
- 标记
- This HNRNPA1 protein is labelled with Strep Tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- 序列
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MSKSESPKEP EQLRKLFIGG LSFETTDESL RSHFEQWGTL TDCVVMRDPN TKRSRGFGFV TYATVEEVDA AMNARPHKVD GRVVEPKRAV SREDSQRPGA HLTVKKIFVG GIKEDTEEHH LRDYFEQYGK IEVIEIMTDR GSGKKRGFAF VTFDDHDSVD KIVIQKYHTV NGHNCEVRKA LSKQEMASAS SSQRGRSGSG NFGGGRGGGF GGNDNFGRGG NFSGRGGFGG SRGGGGYGGS GDGYNGFGND GSNFGGGGSY NDFGNYNNQS SNFGPMKGGN FGGRSSGPYG GGGQYFAKPR NQGGYGGSSS SSSYGSGRRF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - 产品特性
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 内毒素水平
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product HNRNPA1 蛋白
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Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) (AA 1-320) protein (His tag)
HNRNPA1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) (AA 1-320) protein (GST tag)HNRNPA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) (AA 1-372) protein (Strep Tag)Crystallography grade HNRNPA1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)HNRNPA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)HNRNPA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- HNRNPA1 (Heterogeneous Nuclear Ribonucleoprotein A1 (HNRNPA1))
- 别名
- Hnrnpa1 (HNRNPA1 产品)
- 别名
- HNRPA1 Protein, HNRPA1L3 Protein, hnRNP A1 Protein, hnRNP-A1 Protein, hnrpa1 Protein, TPT1P Protein, HNRNPA1 Protein, D15Ertd119e Protein, Hdp Protein, Hnrpa1 Protein, hnrnp-A1 Protein, ROA1 Protein, zgc:66127 Protein, heterogeneous nuclear ribonucleoprotein A1 Protein, heterogeneous nuclear ribonucleoprotein A1b Protein, Heterogeneous nuclear ribonucleoprotein A1 Protein, heterogeneous nuclear ribonucleoprotein A1 S homeolog Protein, ribonucleoprotein A1a Protein, heterogeneous nuclear ribonucleoprotein A1-like Protein, heterogeneous nuclear ribonucleoprotein A1a Protein, HNRNPA1 Protein, hnrnpa1b Protein, LOC100101226 Protein, roa1 Protein, Hnrnpa1 Protein, hnrnpa1.S Protein, hnrnpa1 Protein, LOC100359118 Protein, hnrnpa1a Protein
- 背景
- Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) (HDP-1) (Helix-destabilizing protein) (Single-strand-binding protein) (Topoisomerase-inhibitor suppressed) (hnRNP core protein A1) [Cleaved into: Heterogeneous nuclear ribonucleoprotein A1, N-terminally processed],FUNCTION: Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and modulation of splice site selection. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. Binds to the IRES and thereby inhibits the translation of the apoptosis protease activating factor APAF1. May bind to specific miRNA hairpins. {ECO:0000250|UniProtKB:P09651}.
- 分子量
- 34.2 kDa
- UniProt
- P49312
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