Angiotensin I Converting Enzyme 1 Protein (AA 35-1264) (His tag)
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- 抗原 See all Angiotensin I Converting Enzyme 1 (ACE) 蛋白
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
- 蛋白类型
- Recombinant
- 产品特性
- AA 35-1264
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This Angiotensin I Converting Enzyme 1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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LDPGLQPGNF SPDEAGAQLF AESYNSSAEV VMFQSTVASW AHDTNITEEN ARRQEEAALV SQEFAEVWGK KAKELYESIW QNFTDSKLRR IIGSIRTLGP ANLPLAQRQQ YNSLLSNMSR IYSTGKVCFP NKTATCWSLD PELTNILASS RSYAKLLFAW EGWHDAVGIP LKPLYQDFTA ISNEAYRQDD FSDTGAFWRS WYESPSFEES LEHIYHQLEP LYLNLHAYVR RALHRRYGDK YVNLRGPIPA HLLGDMWAQS WENIYDMVVP FPDKPNLDVT STMVQKGWNA THMFRVSEEF FTSLGLSPMP PEFWAESMLE KPTDGREVVC HASAWDFYNR KDFRIKQCTR VTMEQLATVH HEMGHVQYYL QYKDLHVSLR RGANPGFHEA IGDVLALSVS TPAHLHKIGL LDHVTNDIES DINYLLKMAL EKIAFLPFGY LVDQWRWGVF SGRTPPSRYN FDWWYLRTKY QGICPPVARN ETHFDAGAKF HIPNVTPYIR YFVSFVLQFQ FHQALCKEAG HQGPLHQCDI YQSAQAGAKL KQVLQAGCSR PWQEVLKDLV GSDALDAKAL LEYFQPVSQW LEEQNQRNGE VLGWPENQWR PPLPDNYPEG IDLETDEAKA DRFVEEYDRT AQVLLNEYAE ANWQYNTNIT IEGSKILLEK STEVSNHTLK YGTRAKTFDV SNFQNSSIKR IIKKLQNLDR AVLPPKELEE YNQILLDMET TYSLSNICYT NGTCMPLEPD LTNMMATSRK YEELLWAWKS WRDKVGRAIL PFFPKYVEFS NKIAKLNGYT DAGDSWRSLY ESDNLEQDLE KLYQELQPLY LNLHAYVRRS LHRHYGSEYI NLDGPIPAHL LGNMWAQTWS NIYDLVAPFP SAPNIDATEA MIKQGWTPRR IFKEADNFFT SLGLLPVPPE FWNKSMLEKP TDGREVVCHP SAWDFYNGKD FRIKQCTSVN MEDLVIAHHE MGHIQYFMQY KDLPVTFREG ANPGFHEAIG DIMALSVSTP KHLYSLNLLS TEGSGYEYDI NFLMKMALDK IAFIPFSYLI DQWRWRVFDG SITKENYNQE WWSLRLKYQG LCPPVPRSQG DFDPGSKFHV PANVPYVRYF VSFIIQFQFH EALCRAAGHT GPLHKCDIYQ SKEAGKLLAD AMKLGYSKPW PEAMKLITGQ PNMSASAMMN YFKPLTEWLV TENRRHGETL GWPEYNWAPN TARAEGSTAE SNRVNFLGLY LEPQQARVGQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Ace Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ACE 蛋白
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Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 592-701) protein (GST tag)
ACE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 30-1256) protein (His tag)Crystallography grade ACE 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 30-1232) protein (rho-1D4 tag)Crystallography grade ACE 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 30-1306) protein (rho-1D4 tag)Crystallography grade ACE 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 35-1237) protein (rho-1D4 tag)Crystallography grade ACE 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE) (AA 35-1312) protein (rho-1D4 tag)Crystallography grade ACE 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
- 别名
- Ace (ACE 产品)
- 别名
- ACE1 Protein, CD143 Protein, DCP Protein, DCP1 Protein, ICH Protein, MVCD3 Protein, AW208573 Protein, Dcp1 Protein, StsRR92 Protein, dcp Protein, ace1 Protein, dcp1 Protein, xace Protein, cd143 Protein, ACE Protein, angiotensin I converting enzyme Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 Protein, angiotensin-converting enzyme Protein, angiotensin I converting enzyme (peptidyl-dipeptidase A) 3 Protein, angiotensin-converting enzyme-like Protein, ACE Protein, Ace Protein, ace Protein, CpipJ_CPIJ009106 Protein, ACE3 Protein, LOC101824864 Protein
- 背景
- Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm. {ECO:0000269|PubMed:15665832, ECO:0000269|PubMed:7753170, ECO:0000269|PubMed:8642790}.
- 分子量
- 142.8 kDa Including tag.
- UniProt
- P09470
- 途径
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
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