Laminin gamma 1 Protein (AA 34-1607) (His tag)
-
- 抗原 See all Laminin gamma 1 (LAMC1) 蛋白
- Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 34-1607
-
宿主
- 小鼠
-
资源
- Insect Cells
- 标记
- This Laminin gamma 1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
-
AMDECADEGG RPQRCMPEFV NAAFNVTVVA TNTCGTPPEE YCVQTGVTGV TKSCHLCDAG QQHLQHGAAF LTDYNNQADT TWWQSQTMLA GVQYPNSINL TLHLGKAFDI TYVRLKFHTS RPESFAIYKR TREDGPWIPY QYYSGSCENT YSKANRGFIR TGGDEQQALC TDEFSDISPL TGGNVAFSTL EGRPSAYNFD NSPVLQEWVT ATDIRVTLNR LNTFGDEVFN EPKVLKSYYY AISDFAVGGR CKCNGHASEC VKNEFDKLMC NCKHNTYGVD CEKCLPFFND RPWRRATAES ASESLPCDCN GRSQECYFDP ELYRSTGHGG HCTNCRDNTD GAKCERCREN FFRLGNTEAC SPCHCSPVGS LSTQCDSYGR CSCKPGVMGD KCDRCQPGFH SLTEAGCRPC SCDLRGSTDE CNVETGRCVC KDNVEGFNCE RCKPGFFNLE SSNPKGCTPC FCFGHSSVCT NAVGYSVYDI SSTFQIDEDG WRVEQRDGSE ASLEWSSDRQ DIAVISDSYF PRYFIAPVKF LGNQVLSYGQ NLSFSFRVDR RDTRLSAEDL VLEGAGLRVS VPLIAQGNSY PSETTVKYIF RLHEATDYPW RPALSPFEFQ KLLNNLTSIK IRGTYSERTA GYLDDVTLQS ARPGPGVPAT WVESCTCPVG YGGQFCETCL PGYRRETPSL GPYSPCVLCT CNGHSETCDP ETGVCDCRDN TAGPHCEKCS DGYYGDSTLG TSSDCQPCPC PGGSSCAIVP KTKEVVCTHC PTGTAGKRCE LCDDGYFGDP LGSNGPVRLC RPCQCNDNID PNAVGNCNRL TGECLKCIYN TAGFYCDRCK EGFFGNPLAP NPADKCKACA CNPYGTVQQQ SSCNPVTGQC QCLPHVSGRD CGTCDPGYYN LQSGQGCERC DCHALGSTNG QCDIRTGQCE CQPGITGQHC ERCETNHFGF GPEGCKPCDC HHEGSLSLQC KDDGRCECRE GFVGNRCDQC EENYFYNRSW PGCQECPACY RLVKDKAAEH RVKLQELESL IANLGTGDDM VTDQAFEDRL KEAEREVTDL LREAQEVKDV DQNLMDRLQR VNSSLHSQIS RLQNIRNTIE ETGILAERAR SRVESTEQLI EIASRELEKA KMAAANVSIT QPESTGEPNN MTLLAEEARR LAERHKQEAD DIVRVAKTAN ETSAEAYNLL LRTLAGENQT ALEIEELNRK YEQAKNISQD LEKQAARVHE EAKRAGDKAV EIYASVAQLT PVDSEALENE ANKIKKEAAD LDRLIDQKLK DYEDLREDMR GKEHEVKNLL EKGKAEQQTA DQLLARADAA KALAEEAAKK GRSTLQEAND ILNNLKDFDR RVNDNKTAAE EALRRIPAIN RTIAEANEKT REAQLALGNA AADATEAKNK AHEAERIASA VQKNATSTKA DAERTFGEVT DLDNEVNGML RQLEEAENEL KRKQDDADQD MMMAGMASQA AQEAELNARK AKNSVSSLLS QLNNLLDQLG QLDTVDLNKL NEIEGSLNKA KDEMKASDLD RKVSDLESEA RKQEAAIMDY NRDIAEIIKD IHNLEDIKKT LPTGCFNTPS IEKP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
-
- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Lamc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
-
Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product LAMC1 蛋白
-
Laminin, gamma 1 (LAMC1) (AA 1-38) protein (GST tag)
LAMC1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Laminin, gamma 1 (LAMC1) (AA 34-1609) protein (His tag)Crystallography grade LAMC1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Laminin, gamma 1 (LAMC1) proteinLAMC1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Laminin, gamma 1 (LAMC1) proteinLAMC1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
Laminin, gamma 1 (LAMC1) protein (Myc-DYKDDDDK Tag)LAMC1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
-
Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
-
- 抗原
- Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))
- 别名
- Lamc1 (LAMC1 产品)
- 别名
- lamb2 Protein, Lamb2 Protein, B2e Protein, sly Protein, LAMB2 Protein, putative laminin gamma-1 chain Protein, laminin subunit gamma 1 Protein, laminin, gamma 1 Protein, Smp_163810 Protein, lamc1 Protein, Lamc1 Protein, LAMC1 Protein
- 背景
- Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
- 分子量
- 175.0 kDa Including tag.
- UniProt
- P02468
-
