NPC1 Protein (AA 23-269) (His tag)
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- 抗原 See all NPC1 蛋白
- NPC1 (Niemann-Pick Disease, Type C1 (NPC1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-269
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宿主
- 小鼠
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资源
- Insect Cells
- 标记
- This NPC1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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QSCVWYGECG IATGDKRYNC KYSGPPKPLP KDGYDLVQEL CPGLFFDNVS LCCDIQQLQT LKSNLQLPLQ FLSRCPSCFY NLMTLFCELT CSPHQSQFLN VTATEDYFDP KTQENKTNVK ELEYFVGQSF ANAMYNACRD VEAPSSNEKA LGLLCGRDAR ACNATNWIEY MFNKDNGQAP FTIIPVFSDL SILGMEPMRN ATKGCNESVD EVTGPCSCQD CSIVCGPKPQ PPPPPMPWRI WGLDAMY
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Npc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product NPC1 蛋白
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Niemann-Pick Disease, Type C1 (NPC1) (AA 151-250) protein (GST tag)
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Niemann-Pick Disease, Type C1 (NPC1) protein (His tag,DYKDDDDK Tag)NPC1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Niemann-Pick Disease, Type C1 (NPC1) (AA 372-620) protein (His tag)Crystallography grade NPC1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Niemann-Pick Disease, Type C1 (NPC1) (AA 372-621) protein (His tag)Crystallography grade NPC1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Niemann-Pick Disease, Type C1 (NPC1) (AA 23-269) protein (His tag)Crystallography grade NPC1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Niemann-Pick Disease, Type C1 (NPC1) (AA 854-1098) protein (His tag)Crystallography grade NPC1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Niemann-Pick Disease, Type C1 (NPC1) (AA 854-1076) protein (His tag)Crystallography grade NPC1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Niemann-Pick Disease, Type C1 (NPC1) (AA 23-1278) protein (rho-1D4 tag)Crystallography grade NPC1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Niemann-Pick Disease, Type C1 (NPC1) (AA 23-1277) protein (rho-1D4 tag)Crystallography grade NPC1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- NPC1 (Niemann-Pick Disease, Type C1 (NPC1))
- 别名
- Npc1 (NPC1 产品)
- 别名
- NPC Protein, 9130221N23Rik Protein, Gm243 Protein, Cdig2 Protein, im:7149020 Protein, wu:fb53a12 Protein, wu:fc29a12 Protein, A430089E03Rik Protein, C85354 Protein, D18Ertd139e Protein, D18Ertd723e Protein, lcsd Protein, nmf164 Protein, spm Protein, NPC intracellular cholesterol transporter 1 Protein, NPC1 like intracellular cholesterol transporter 1 Protein, Niemann-Pick disease, type C1 Protein, Niemann-Pick C1 protein Protein, NPC1 Protein, Npc1l1 Protein, Npc1 Protein, npc1 Protein, LOC579887 Protein
- 背景
- Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals.
- 分子量
- 28.6 kDa Including tag.
- UniProt
- O35604
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