Histone H2A Protein (AA 2-130) (His tag)
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- 抗原 See all Histone H2A 蛋白
- Histone H2A
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-130
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Histone H2A protein is labelled with His tag.
- 应用范围
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- 序列
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SGRGKQGGKA RAKAKTRSSR AGLQFPVGRV HRLLRKGNYS ERVGAGAPVY LAAVLEYLTA EILELAGNAA RDNKKTRIIP RHLQLAIRND EELNKLLGRV TIAQGGVLPN IQAVLLPKKT ESHHKPKGK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Hist1h2af Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product Histone H2A 蛋白
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Histone H2A (AA 2-130) protein (His tag)
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Histone H2A
- 别名
- Hist1h2af (Histone H2A 产品)
- 别名
- 5499 Protein, CG5499 Protein, Dmel\\CG5499 Protein, H2A Protein, H2A.F/Z Protein, H2A.X Protein, H2A.Z Protein, H2AV Protein, H2AV_DROM Protein, H2AX Protein, H2AZ Protein, H2Av Protein, H2AvD Protein, H2a.V Protein, H2av Protein, H2avD Protein, HIS Protein, HIS2AV Protein, HIS2AVD Protein, His Protein, His2 Protein, His2AV Protein, His2AvD Protein, HisH2Av Protein, Hist Protein, Hist2av Protein, gamma-H2Av Protein, gamma-HIS2AV Protein, gamma-His2Av Protein, gammaH2Av Protein, h2AvD Protein, his Protein, l(3)05146 Protein, l(3)810 Protein, l(3)97Dd Protein, l(3)L1602 Protein, DDBDRAFT_0205974 Protein, DDBDRAFT_0216271 Protein, DDB_0205974 Protein, DDB_0216271 Protein, RGD1564767 Protein, H2a-221 Protein, H2A-III Protein, HIST1H2AA Protein, Histone H2A variant Protein, putative histone H2A Protein, histone H2A Protein, histone cluster 1 H2A family member F Protein, histone H2A-like Protein, histone cluster 1, H2af Protein, histone2A1 Protein, histone cluster 1, H2A, III Protein, histone cluster 1, H2ac Protein, His2Av Protein, LMJF_17_0280 Protein, H2AX Protein, Hist1h2af Protein, LOC100425378 Protein, his2a1 Protein, HIST1H2A3 Protein, HIST1H2AC Protein, h2a-A Protein
- 背景
- Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- 分子量
- 15.0 kDa Including tag.
- UniProt
- Q8CGP5
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