Glucagon Receptor Protein (GCGR) (AA 26-477) (rho-1D4 tag)
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- 抗原 See all Glucagon Receptor (GCGR) 蛋白
- Glucagon Receptor (GCGR)
- 蛋白类型
- Recombinant
- 产品特性
- AA 26-477
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宿主
- 人
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资源
- Insect Cells
- 标记
- This Glucagon Receptor protein is labelled with rho-1D4 tag.
- 应用范围
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- 序列
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AQVMDFLFEK WKLYGDQCHH NLSLLPPPTE LVCNRTFDKY SCWPDTPANT TANISCPWYL PWHHKVQHRF VFKRCGPDGQ WVRGPRGQPW RDASQCQMDG EEIEVQKEVA KMYSSFQVMY TVGYSLSLGA LLLALAILGG LSKLHCTRNA IHANLFASFV LKASSVLVID GLLRTRYSQK IGDDLSVSTW LSDGAVAGCR VAAVFMQYGI VANYCWLLVE GLYLHNLLGL ATLPERSFFS LYLGIGWGAP MLFVVPWAVV KCLFENVQCW TSNDNMGFWW ILRFPVFLAI LINFFIFVRI VQLLVAKLRA RQMHHTDYKF RLAKSTLTLI PLLGVHEVVF AFVTDEHAQG TLRSAKLFFD LFLSSFQGLL VAVLYCFLNK EVQSELRRRW HRWRLGKVLW EERNTSNHRA SSSPGHGPPS KELQFGRGGG SQDSSAETPL AGGLPRLAES PF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human GCGR Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product GCGR 蛋白
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Glucagon Receptor (GCGR) (AA 26-136) protein (His tag)
Crystallography grade GCGR 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Glucagon Receptor (GCGR) (AA 27-137) protein (His tag)Crystallography grade GCGR 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Glucagon Receptor (GCGR) (AA 27-485) protein (rho-1D4 tag)Crystallography grade GCGR 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Glucagon Receptor (GCGR) (AA 26-136) protein (His tag)GCGR 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Glucagon Receptor (GCGR)
- 别名
- GCGR (GCGR 产品)
- 别名
- GCGR Protein, GGR Protein, GR Protein, glucagon receptor Protein, GCGR Protein, Gcgr Protein
- 背景
- G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system. {ECO:0000269|PubMed:19657311, ECO:0000269|PubMed:22908259, ECO:0000269|PubMed:23863937, ECO:0000269|PubMed:7507321, ECO:0000269|PubMed:9287038}.
- 分子量
- 52.4 kDa Including tag.
- UniProt
- P47871
- 途径
- Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process
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