PLAP Protein (AA 23-506) (rho-1D4 tag)
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- 抗原 See all PLAP (ALPP) 蛋白
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- 蛋白类型
- Recombinant
- 产品特性
- AA 23-506
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宿主
- 人
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资源
- Insect Cells
- 标记
- This PLAP protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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IIPVEEENPD FWNREAAEAL GAAKKLQPAQ TAAKNLIIFL GDGMGVSTVT AARILKGQKK DKLGPEIPLA MDRFPYVALS KTYNVDKHVP DSGATATAYL CGVKGNFQTI GLSAAARFNQ CNTTRGNEVI SVMNRAKKAG KSVGVVTTTR VQHASPAGTY AHTVNRNWYS DADVPASARQ EGCQDIATQL ISNMDIDVIL GGGRKYMFRM GTPDPEYPDD YSQGGTRLDG KNLVQEWLAK RQGARYVWNR TELMQASLDP SVTHLMGLFE PGDMKYEIHR DSTLDPSLME MTEAALRLLS RNPRGFFLFV EGGRIDHGHH ESRAYRALTE TIMFDDAIER AGQLTSEEDT LSLVTADHSH VFSFGGYPLR GSSIFGLAPG KARDRKAYTV LLYGNGPGYV LKDGARPDVT ESESGSPEYR QQSAVPLDEE THAGEDVAVF ARGPQAHLVH GVQEQTFIAH VMAFAACLEP YTACDLAPPA GTTD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human ALPP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin-free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product ALPP 蛋白
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Placental Alkaline Phosphatase (ALPP) (AA 1-535) protein (GST tag)
ALPP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Placental Alkaline Phosphatase (ALPP) (AA 23-506) (Active) protein (His tag)ALPP 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % by SDS - PAGE SDS Active
Placental Alkaline Phosphatase (ALPP) protein (Myc-DYKDDDDK Tag)ALPP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- 别名
- ALPP (ALPP 产品)
- 别名
- ECK0378 Protein, JW0374 Protein, psiA Protein, ALP Protein, PALP Protein, PLAP Protein, PLAP-1 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, ALPI Protein, Actn2lp Protein, Alp Protein, bacterial alkaline phosphatase Protein, alkaline phosphatase, placental Protein, alkaline phosphatase, placental type Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, alkaline phosphatase, intestinal Protein, PDZ and LIM domain 3 Protein, phoA Protein, ALPP Protein, LOC100436725 Protein, CCL27 Protein, alpl Protein, alp Protein, ALPI Protein, Pdlim3 Protein, Alpp Protein
- 分子量
- 53.9 kDa Including tag.
- UniProt
- P05187
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