PLAP Protein (AA 23-506) (His tag)
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- 抗原 See all PLAP (ALPP) 蛋白
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 23-506
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This PLAP protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS)
- 序列
- ADLMIIPVEE ENPDFWNREA AEALGAAKKL QPAQTAAKNL IIFLGDGMGV STVTAARILK GQKKDKLGPE LPLAMDRFPY VALSKTYNVD KHVPDSGATA TAYLCGVKGN FQTIGLSAAA RFNQCNTTRG NEVISVMNRA KKAGKSVGVV TTTRVQHASP AGTYAHTVNR NWYSDADVPA SARQEGCQDI ATQLISNMDI DVILGGGRKY MFRMGTPDPE YPDDYSQGGT RLDGKNLVQE WLAKRQGARY VWNRTELMQA SLDPSVTHLM GLFEPGDMKY EIHRDSTLDP SLMEMTEAAL RLLSRNPRGF FLFVEGGRID HGHHESRAYR ALTETIMFDD AIERAGQLTS EEDTLSLVTA DHSHVFSFGG YPLRGSSIFG LAPGKARDRK AYTVLLYGNG PGYVLKDGAR PDVTESESGS PEYRQQSAVP LDEETHAGED VAVFARGPQA HLVHGVQEQT FIAHVMAFAA CLEPYTACDL APPAGTTDHH HHHH
- 纯度
- > 95 % by SDS - PAGE
- 内毒素水平
- < 1.0 EU per 1 microgram of protein (determined by LAL method)
- Biological Activity Comment
- Specific activity is > 2,500 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.
- Top Product
- Discover our top product ALPP 蛋白
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Placental Alkaline Phosphatase (ALPP) (AA 1-535) protein (GST tag)
ALPP 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Placental Alkaline Phosphatase (ALPP) (AA 23-506) protein (rho-1D4 tag)Crystallography grade ALPP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Placental Alkaline Phosphatase (ALPP) protein (Myc-DYKDDDDK Tag)ALPP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Bioactivity Validated
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Liquid. In Phosphate Buffered Saline ( pH 7.4) containing 10 % glycerol.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
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- 抗原
- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
- 别名
- Alkaline phosphatase, placental (ALPP 产品)
- 别名
- ECK0378 Protein, JW0374 Protein, psiA Protein, ALP Protein, PALP Protein, PLAP Protein, PLAP-1 Protein, ILC Protein, CTACK Protein, ESkine Protein, Akp2 Protein, alp Protein, zgc:56672 Protein, DDBDRAFT_0205491 Protein, DDBDRAFT_0231570 Protein, DDB_0205491 Protein, DDB_0231570 Protein, ALPI Protein, Actn2lp Protein, Alp Protein, bacterial alkaline phosphatase Protein, alkaline phosphatase, placental Protein, alkaline phosphatase, placental type Protein, C-C motif chemokine ligand 27 Protein, alkaline phosphatase, liver/bone/kidney Protein, alkaline phosphatase Protein, alkaline phosphatase, intestinal Protein, PDZ and LIM domain 3 Protein, phoA Protein, ALPP Protein, LOC100436725 Protein, CCL27 Protein, alpl Protein, alp Protein, ALPI Protein, Pdlim3 Protein, Alpp Protein
- 背景
- ALPP, also known as alkaline phosphatase, placental, is a family of dimeric metalloenzymes catalyzing the hydrolysis of monoesters of phosphoric acid. Also, this protein catalyzes a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. It occurs widely in nature, and are found in many organisms from Escherichia coli to man. Most alkaline phosphatases are homodimeric enzymes and each catalytic site contains three metal ions (two Zn and one Mg), that are necessary for enzymatic activity. Recombinant human ALPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
- 分子量
- 53.9kDa (494aa) 50-70KDa (SDS-PAGE under reducing conditions).
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