YBX1 Protein (AA 2-324) (His tag)
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- 抗原 See all YBX1 蛋白
- YBX1 (Y Box Binding Protein 1 (YBX1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-324
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宿主
- 人
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资源
- Insect Cells
- 标记
- This YBX1 protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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SSEAETQQPP AAPPAAPALS AADTKPGTTG SGAGSGGPGG LTSAAPAGGD KKVIATKVLG TVKWFNVRNG YGFINRNDTK EDVFVHQTAI KKNNPRKYLR SVGDGETVEF DVVEGEKGAE AANVTGPGGV PVQGSKYAAD RNHYRRYPRR RGPPRNYQQN YQNSESGEKN EGSESAPEGQ AQQRRPYRRR RFPPYYMRRP YGRRPQYSNP PVQGEVMEGA DNQGAGEQGR PVRQNMYRGY RPRFRRGPPR QRQPREDGNE EDKENQGDET QGQQPPQRRY RRNFNYRRRR PENPKPQDGK ETKAADPPAE NSSAPEAEQG GAE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human YBX1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product YBX1 蛋白
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Y Box Binding Protein 1 (YBX1) (AA 1-324) protein (GST tag)
YBX1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Y Box Binding Protein 1 (YBX1) (AA 1-324) protein (His tag)YBX1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Y Box Binding Protein 1 (YBX1) (AA 51-139) protein (GST tag)YBX1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Y Box Binding Protein 1 (YBX1) proteinYBX1 宿主: Borrelia burgdorferi 宿主: 大肠杆菌（E. Coli） Recombinant WB, ELISA
Y Box Binding Protein 1 (YBX1) (AA 2-322) protein (His tag)YBX1 宿主: 小鼠 宿主: 酵母菌 Recombinant > 90 % SDS
Y Box Binding Protein 1 (YBX1) protein (Myc-DYKDDDDK Tag)YBX1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- YBX1 (Y Box Binding Protein 1 (YBX1))
- 别名
- YBX1 (YBX1 产品)
- 别名
- BP-8 Protein, CSDA2 Protein, CSDB Protein, DBPB Protein, MDR-NF1 Protein, NSEP-1 Protein, NSEP1 Protein, YB-1 Protein, YB1 Protein, Byb1 Protein, Cbfa Protein, Dbpb Protein, Ef1a Protein, Msy1 Protein, Nsep1 Protein, Yb1 Protein, bp-8 Protein, csda2 Protein, csdb Protein, dbpb Protein, frgy1 Protein, mdr-nf1 Protein, nsep-1 Protein, nsep1 Protein, yb1 Protein, 1700102N10Rik Protein, C79409 Protein, EF1A Protein, MSY1 Protein, dbpB Protein, mYB-1a Protein, nuclease sensitive element binding protein 1 Protein, Y-box binding protein 1 Protein, Y box binding protein 1 Protein, Y-box binding protein 1 S homeolog Protein, nuclease-sensitive element-binding protein 1 Protein, Y box protein 1 Protein, nsep1 Protein, YBX1 Protein, Ybx1 Protein, ybx1.S Protein, LOC479011 Protein
- 背景
- Mediates pre-mRNA alternative splicing regulation. Binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Regulates the transcription of numerous genes. Its transcriptional activity on the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7'. Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes. Promotes separation of DNA strands that contain mismatches or are modified by cisplatin. Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro). May play a role in DNA repair. Component of the CRD-mediated complex that promotes MYC mRNA stability. Binds preferentially to the 5'-[CU]CUGCG-3' motif in vitro. {ECO:0000250, ECO:0000269|PubMed:10817758, ECO:0000269|PubMed:11698476, ECO:0000269|PubMed:12604611, ECO:0000269|PubMed:14718551, ECO:0000269|PubMed:18335541, ECO:0000269|PubMed:18809583, ECO:0000269|PubMed:19029303, ECO:0000269|PubMed:19483673}., The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation.
- 分子量
- 36.7 kDa Including tag.
- UniProt
- P67809
- 途径
- Regulation of Muscle Cell Differentiation
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