Vinculin Protein (VCL) (AA 2-1134) (His tag)
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- 抗原 See all Vinculin (VCL) 蛋白
- Vinculin (VCL)
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-1134
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宿主
- 人
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资源
- Insect Cells
- 标记
- This Vinculin protein is labelled with His tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- 序列
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PVFHTRTIES ILEPVAQQIS HLVIMHEEGE VDGKAIPDLT APVAAVQAAV SNLVRVGKET VQTTEDQILK RDMPPAFIKV ENACTKLVQA AQMLQSDPYS VPARDYLIDG SRGILSGTSD LLLTFDEAEV RKIIRVCKGI LEYLTVAEVV ETMEDLVTYT KNLGPGMTKM AKMIDERQQE LTHQEHRVML VNSMNTVKEL LPVLISAMKI FVTTKNSKNQ GIEEALKNRN FTVEKMSAEI NEIIRVLQLT SWDEDAWASK DTEAMKRALA SIDSKLNQAK GWLRDPSASP GDAGEQAIRQ ILDEAGKVGE LCAGKERREI LGTCKMLGQM TDQVADLRAR GQGSSPVAMQ KAQQVSQGLD VLTAKVENAA RKLEAMTNSK QSIAKKIDAA QNWLADPNGG PEGEEQIRGA LAEARKIAEL CDDPKERDDI LRSLGEISAL TSKLADLRRQ GKGDSPEARA LAKQVATALQ NLQTKTNRAV ANSRPAKAAV HLEGKIEQAQ RWIDNPTVDD RGVGQAAIRG LVAEGHRLAN VMMGPYRQDL LAKCDRVDQL TAQLADLAAR GEGESPQARA LASQLQDSLK DLKARMQEAM TQEVSDVFSD TTTPIKLLAV AATAPPDAPN REEVFDERAA NFENHSGKLG ATAEKAAAVG TANKSTVEGI QASVKTAREL TPQVVSAARI LLRNPGNQAA YEHFETMKNQ WIDNVEKMTG LVDEAIDTKS LLDASEEAIK KDLDKCKVAM ANIQPQMLVA GATSIARRAN RILLVAKREV ENSEDPKFRE AVKAASDELS KTISPMVMDA KAVAGNISDP GLQKSFLDSG YRILGAVAKV REAFQPQEPD FPPPPPDLEQ LRLTDELAPP KPPLPEGEVP PPRPPPPEEK DEEFPEQKAG EVINQPMMMA ARQLHDEARK WSSKPGIPAA EVGIGVVAEA DAADAAGFPV PPDMEDDYEP ELLLMPSNQP VNQPILAAAQ SLHREATKWS SKGNDIIAAA KRMALLMAEM SRLVRGGSGT KRALIQCAKD IAKASDEVTR LAKEVAKQCT DKRIRTNLLQ VCERIPTIST QLKILSTVKA TMLGRTNISD EESEQATEML VHNAQNLMQS VKETVREAEA ASIKIRTDAG FTLRWVRKTP WYQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human VCL Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product VCL 蛋白
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Vinculin (VCL) (AA 2-235) protein (His tag)
VCL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vinculin (VCL) (AA 777-1066) protein (GST tag)VCL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Vinculin (VCL) (AA 1-1066) protein (GST tag)VCL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vinculin (VCL) protein (His tag)VCL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Vinculin (VCL) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)VCL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Vinculin (VCL)
- 别名
- VCL (VCL 产品)
- 别名
- CMD1W Protein, CMH15 Protein, MV Protein, MVCL Protein, VCL Protein, im:6902790 Protein, hm:zehn2160 Protein, DDBDRAFT_0186762 Protein, DDBDRAFT_0232320 Protein, DDB_0186762 Protein, DDB_0232320 Protein, 9430097D22 Protein, AA571387 Protein, AI462105 Protein, AW545629 Protein, CG3299 Protein, CT11081 Protein, Dmel\\CG3299 Protein, Dmvincp Protein, EG:103B4.1 Protein, Vin Protein, Vin2EF Protein, vinc Protein, LOC100228062 Protein, vcl-A Protein, vinculin Protein, vinculin a Protein, vinculin A Protein, Vinculin Protein, vinculin S homeolog Protein, VCL Protein, vcla Protein, vinA Protein, Vcl Protein, Vinc Protein, CpipJ_CPIJ013873 Protein, Bm1_37935 Protein, vcl Protein, vcl.S Protein
- 背景
- Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. {ECO:0000269|PubMed:20484056}.
- 分子量
- 124.6 kDa Including tag.
- UniProt
- P18206
- 途径
- Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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